Immunofluorescence (A-C) and IHC (D-G) analysis of XAGE-1b. Immunofluorescence (A-C) and IHC (D-G) analysis of XAGE-1b. (A) Expression of XAGE-1b in 293T cells transfected with pcDNA3.1/XAGE-1b (green) by flow cytometry. Solid line, empty vector. (B) Fluorescence microscopy analysis. (C) Nuclei are shown stained with PI (red). XAGE-1b derived fluorescence is merged with the corresponding PI recordings. (D), (E), (F) and (G) are IHC analysis of testis, lung cancer, hepatocellular carcinoma and gastric cancer, respectively. Magnification 400x. Shuichiro Sato et al. Cancer Immun 2007;7:5 Copyright © 2007 by Shuichiro Sato