Influence of ejaculatory abstinence on seminal total antioxidant capacity and sperm membrane lipid peroxidation  Paul B. Marshburn, M.D., Allie Giddings, M.D., Stephanie Causby, M.S., Michelle L. Matthews, M.D., Rebecca S. Usadi, M.D., Nury Steuerwald, Ph.D., Bradley S. Hurst, M.D.  Fertility and Sterility  Volume 102, Issue 3, Pages 705-710 (September 2014) DOI: 10.1016/j.fertnstert.2014.05.039 Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Comparison of the total antioxidant capacity (TAC) in seminal fluid after 1 day (EA1) vs. 4 days (EA4) of ejaculatory abstinence. The counted photons per minute measures the total antioxidant capacity [TAC]; (see Materials and Methods). TAC determinations were compared within a given male subject by a two-tailed t test, with a man serving as his own control from EA1 to EA4. ∗P<.02. Fertility and Sterility 2014 102, 705-710DOI: (10.1016/j.fertnstert.2014.05.039) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Comparison of the sperm membrane lipid peroxidation (LPO) after 1 day (EA1) vs. 4 days (EA4) of ejaculatory abstinence. The level of malondialdehyde (nM/h) measures the degree of sperm membrane lipid peroxidation (see Materials and Methods). LPO determinations were compared within a given male subject by a two-tailed t test, with a man serving as his own control from EA1 to EA4. P=.98. Fertility and Sterility 2014 102, 705-710DOI: (10.1016/j.fertnstert.2014.05.039) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions