Comparison of PQ-CSF and IQ-CSF RT-QuIC analyses of individual sCJD and negative-control CSF samples. Comparison of PQ-CSF and IQ-CSF RT-QuIC analyses.

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Comparison of PQ-CSF and IQ-CSF RT-QuIC analyses of individual sCJD and negative-control CSF samples. Comparison of PQ-CSF and IQ-CSF RT-QuIC analyses of individual sCJD and negative-control CSF samples. CSFs were tested with either the IQ-CSF (red circles) or PQ-CSF (blue triangles) conditions. The latter data have been reported previously (21). Traces from 2 non-neurological control (NNC) samples analyzed with the IQ-CSF conditions are reported in panel l (green circles). Due to technical issues, traces in panels i and k end at 45 h. Nevertheless, using both conditions, these samples were called positive based on the criteria established in Materials and Methods. Symbols indicate the means from three or four replicate wells. Color-matched fractions indicate the number of positive wells out of the total number of replicate reactions for each sample. Christina D. Orrú et al. mBio 2015; doi:10.1128/mBio.02451-14