Impact of FRC-specific Myd88 ablation on omental FALC organization.

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Impact of FRC-specific Myd88 ablation on omental FALC organization. Impact of FRC-specific Myd88 ablation on omental FALC organization. (A) Confocal microscopic analysis of omental FALCs in 8- to 10-week-old Ccl19-EYFP mice (top) and mice lacking MYD88 expression in FRCs (bottom) using the indicated markers. Scale bar, 100 μm. (B) Flow cytometric analysis of canonical FRC marker expression on CD45−PDPN+EYFP+ cells from the omentum of the indicated mouse strains; isotype control staining is indicated in gray. ICAM, intercellular adhesion molecule; VCAM, vascular cell adhesion molecule. (C to E) Enumeration of FRCs by flow cytometry. Representative dot plots of PDPN+EYFP+ cells (C) and frequency (D) and absolute numbers (E) of PDPN+EYFP+ cells in the omentum of the indicated mouse strains. (F) Quantification of omental FALC numbers and size in whole mount stains; number of FALCs per square millimeter (left) and FALC-covered area in the omentum (right). Enumeration of hematopoietic cells (G) and B cell subsets (H) in mice lacking MYD88 expression in FRCs or in Cre-negative littermates (Ctrl) by flow cytometric analysis. Data are from one representative sample with five to eight mice (A to C) or pooled data from two independent experiments [n = 5 to 8 mice per group, with bars in (D) to (H) representing mean values ± SEM]. Statistical analysis was performed using Student’s t test with *P < 0.05 and **P < 0.01. Christian Perez-Shibayama et al. Sci. Immunol. 2018;3:eaar4539 Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works