Inhibition of the GSK3 activity attenuates the morphology and sphere-forming ability of iCSCs. Inhibition of the GSK3 activity attenuates the morphology.

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Inhibition of the GSK3 activity attenuates the morphology and sphere-forming ability of iCSCs. Inhibition of the GSK3 activity attenuates the morphology and sphere-forming ability of iCSCs. A, Effects of single or double GSK3α and GSK3β knockdowns on the cell morphology (left) and the cell number (right) of parental SW480 cells and 2nd V50-OKS cells 5 days posttransfection (n = 3). *, P < 0.05. B, The morphologic changes (left) and the cell number (right) of parental SW480 cells and 2nd V50-OKS cells with no compound, VPA, and CHIR99021. VPA and CHIR99021 destroyed the dome-shaped colonies of 2nd V50-OKS cells, but did not change the spindle shape of parental SW480 cells (n = 3). *, P < 0.05; **, P < 0.01. C, The sphere-forming ability in parental SW480 cells or 2nd V50-OKS cells with no compound, VPA, and CHIR99021. The number of spheres were counted 10 days after being plated (right; n = 3). *, P < 0.05; **, P < 0.01. Ryo Ishida et al. Mol Cancer Res 2017;15:1455-1466 ©2017 by American Association for Cancer Research