Manhattan plot of 11,628 m/z features comparing participants who developed incident T2D versus those who did not. Manhattan plot of 11,628 m/z features comparing participants who developed incident T2D versus those who did not. The negative log P value was plotted against the m/z features. The x-axis represents m/z of the detected features, ordered in increasing value from 85 (left) to 1,800 (right). A total of seven metabolites significantly differed between the two groups at the threshold of q = 0.05 (above the horizontal gray line). Jinying Zhao et al. Dia Care 2015;38:220-227 ©2015 by American Diabetes Association