Rolf Hoffmann, Shiro Niiyama Journal of Investigative Dermatology

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Steroid Sulfatase in the Human Hair Follicle Concentrates in the Dermal Papilla Rolf Hoffmann, Shiro Niiyama Journal of Investigative Dermatology Volume 117, Issue 6, Pages 1342-1348 (December 2001) DOI: 10.1046/j.0022-202x.2001.01547.x Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Pathways of androgen metabolism in the skin. This scheme illustrates the possible pathways of different androgens in the skin. Journal of Investigative Dermatology 2001 117, 1342-1348DOI: (10.1046/j.0022-202x.2001.01547.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Subunits microdissected from hair follicles. (a) Complete hair follicle from a skin biopsy with dermal papilla, connective tissue sheath and root sheath; (b) root sheath with inner and outer root sheath; (c) connective tissue sheath; (d) dermal papilla. Scale bars: (a–c) 500 µm; (d) 25 µm. Journal of Investigative Dermatology 2001 117, 1342-1348DOI: (10.1046/j.0022-202x.2001.01547.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Conversion of [3H]-DHEAS to DHEA in complete hair follicles Increasing numbers of intact anagen hair follicles were incubated for 24 h in the presence of 5 µM DHEAS (with 2 µCi [1,2,6,7-3H]-DHEAS) in a total volume of 400 µl. Culture supernatants were analyzed by HPLC as described in Materials and Methods and revealed the formation of DHEA as a reaction product. The amount of DHEA formed was directly related to the number of hair follicles used in the incubations. Journal of Investigative Dermatology 2001 117, 1342-1348DOI: (10.1046/j.0022-202x.2001.01547.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Steroid sulfatase immunoreactivity in human hair follicles Immunohistochemical analysis using specific anti-steroid sulfatase antibody has been performed on sections of normal human skin as described in Materials and Methods. The steroid sulfatase immuno reactivity appears as a red stain. All sections were counterstained with hemalaun. A diffuse, but strong immunoreactivity in the dermal papilla continuing over to a much weaker staining in the connecting tissue sheath of human terminal hair follicles in the anagen phase can be seen in (a). In (b) a similar staining can be observed in a vellus hair. Scale bar: 60 μm. Journal of Investigative Dermatology 2001 117, 1342-1348DOI: (10.1046/j.0022-202x.2001.01547.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 5a-DHT is the ultimate reaction product after incubation of dermal papillae with androstenediol. Six dermal papillae were microdissected as described in Materials and Methods and incubated in the presence of 15 nM androstenediol (2 µCi [1,2,6,7-3H]-androstenediol) in a total volume of 400 µl. Culture supernatants were analyzed by HPLC and revealed the formation of 5a-DHT as the ultimate product, indicating 3β-HSD and 5aR activity. The elution time of potential reaction products that were not observed (1: androstenedione; 2: androstanedione; 3: androsterone) is indicated by arrows. Journal of Investigative Dermatology 2001 117, 1342-1348DOI: (10.1046/j.0022-202x.2001.01547.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions