An improved method for endothelial cell seeding on polytetrafluoroethylene small caliber vascular grafts Aviva Schneider, MSc, Raphael N. Melmed, MD,

Slides:

Advertisements

Similar presentations

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

Advertisements

The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.

Shunya Shindo, M. D. , Atsuhiko Takagi, M. D. , Anthony D

In vitro endothelialization of a mesosystemic shunt: A clinical case report Teddy Fischlein, MD, Peter Zilla, MD, PhD, Johann Meinhart, PhD, Rudolf Puschmann,

Aortic injury occurring after minor trauma in ankylosing spondylitis

Heparin-coated catheters and heparin-induced thrombocytopenia

Liposuction-derived human fat used for vascular graft sodding contains endothelial cells and not mesothelial cells as the major cell type Stuart K. Williams,

Development of a “leak-proof,” knitted Dacron vascular prosthesis

Growth and fibrinolytic parameters of human umbilical vein endothelial cells seeded onto cardiovascular grafts Jing Chuan Zhang, MDa,b, Johann Wojta,

Preformed confluent endothelial cell monolayers prevent early platelet deposition on vascular prostheses in baboons Peter A. Schneider, MD, Stephen R.

Somatostatin gene transfer and expression in endothelial cells

A new valvulotome for in situ bypass grafts

Aortic injury occurring after minor trauma in ankylosing spondylitis

An experimental study of eight current arterial prostheses

Origin of endothelial cells that line expanded polytetrafluoroethylene vascular grafts sodded with cells from microvascularized fat Stuart K. Williams,

Nicholas P. Ziats, PhD, James M. Anderson, MD, PhD

Chronic in vitro shear stress stimulates endothelial cell retention on prosthetic vascular grafts and reduces subsequent in vivo neointimal thickness

Elastase-induced matrix degradation in arterial organ cultures: An in vitro model of aneurysmal disease Andrew Wills, BSc, Matthew M. Thompson, MD, FRCS,

Mark F. Fillinger, MD, Susan E. O'Connor, MD, Robert J

An experimental study of eight current arterial prostheses

Mechanisms of arterial graft failure. II

Michael A. Golden, MD, Y. P. Tina Au, PhD, Richard D

J. A. C. Buckels, M. D. , F. R. C. S. , A. G. Nordestgaard, M. D. , S

Seeding of arteriovenous prostheses with homologous endothelium

Confluent durable endothelialization of endarterectomized baboon aorta by early attachment of cultured endothelial cells Peter A. Schneider, MD, Stephen.

Malcolm O. Perry, MD, Richard Kempczinski, MD

The management of massive ultrafiltration distending the aneurysm sac after abdominal aortic aneurysm repair with a polytetrafluoroethylene aortobiiliac.

Heat-denatured albumin-coated Dacron vascular grafts: Physical characteristics and in vivo performance MAJ, MC, USA John D. Rumisek, M.D., Charles E.

Hiromichi Miwa, MD, Takehisa Matsuda, PhD Journal of Vascular Surgery

Heparin-coated catheters and heparin-induced thrombocytopenia

Acute platelet deposition after carotid endarterectomy in sheep: Vein patch compared with gelatin-sealed Dacron and polytetrafluoroethylene patch closure

Jennifer Engle, MD, Hazim J. Safi, MD, Omid Abbassi, MD, Dimitrios C

Tibial bypass grafting for limb salvage with ringed polytetrafluoroethylene prostheses: Results of primary and secondary procedures Hans Schweiger, MD,

Impact of endothelial cell seeding on long-term patency and subendothelial proliferation in a small-caliber highly porous polytetrafluoroethylene graft

Objective tinnitus resulting from internal carotid artery stenosis

Effects of antiplatelet agents in combination with endothelial cell seeding on small- diameter Dacron vascular graft performance in the canine carotid.

Norman A. Shoenfeld, M. D. , Jens Eldrup-Jorgensen, M. D

Malcolm Herring, M.D., Sally Baughman, M.S., John Glover, M.D.

Endothelial cell harvest for seeding vascular prostheses: The influence of technique on cell function, viability, and number Bernard M. Bourke, M.B.B.S.,

Polyurethane vascular prostheses decreases neointimal formation compared with expanded polytetrafluoroethylene Marc G. Jeschke, MD, Valentin Hermanutz,

Recreational weight lifting and aortic dissection: Case report

Calvin B. Ernst, MD Journal of Vascular Surgery

Ruptured abdominal aortic aneurysm: A population-based study

Changes in the cooling rate and medium improve the vascular function in cryopreserved porcine femoral arteries Montserrat Rigol, PhD, Magda Heras, MD,

Cardiovascular surgery—The rocket and its stars: Presidential address

Endothelial seeding of polytetrafluoroethylene femoral popliteal bypasses: The failure of low-density seeding to improve patency Malcolm Herring, MD,

Differential effects of a gram-negative and a gram-positive infection on autogenous and prosthetic grafts Kevin J. Geary, MD, Zygmunt M. Tomkiewicz,

Composite sequential bypasses to the ankle and beyond for limb salvage

Adult human endothelial cell enzymatic harvesting

Research and research funding in vascular surgery

Endothelial cell seeding of a new PTFE vascular prosthesis

J.Dennis Baker, MD Journal of Vascular Surgery

Journal of Vascular Surgery Volume 12, Issue 6, Pages (December 1990)

A simple technique to protect subcutaneous vein grafts

Prevention of graft infection by bonding of gentamycin to Dacron prostheses Axel Haverich, MD, Stephan Hirt, MD, Matthias Karck, MD, Francesco Siclari,

Lipid uptake in expanded polytetrafluoroethylene vascular grafts

Cells derived from omental fat tissue and used for seeding vascular prostheses are not endothelial in origin Michel J.T. Visser, MD, J.Hajo van Bockel,

Reduction in deposition of indium 111—labeled platelets after autologous endothelial cell seeding of Dacron aortic bifurcation grafts in humans: A preliminary.

Biochemical analysis of a human humoral fibroblast inhibitory factor associated with impaired vascular prosthetic graft incorporation Jacob Schneiderman,

Acute aortic occlusion – Factors that influence outcome

The effects of endothelial injury on smooth muscle cell proliferation

Monitoring vascular surgical performance

Glenn C. Hunter, M.D., Stan N. Carson, M.D.

Salvage of exposed arteriovenous hemodialysis fistulas

William C. Quist, MD, PhD, Frank W. LoGerfo, MD

D. Emerick Szilagyi, MD—An appreciation

Presidential address: The second-generation vascular surgeon

George D. Lilly 1906–1988 Journal of Vascular Surgery

James A. DeWeese, MD Journal of Vascular Surgery

Endovascular repair of two abdominal aortic aneurysms

Presentation transcript:

An improved method for endothelial cell seeding on polytetrafluoroethylene small caliber vascular grafts Aviva Schneider, MSc, Raphael N. Melmed, MD, Herzl Schwalb, PhD, Matthias Karck, MD, Israel Vlodavsky, PhD, Gideon Uretzky, MD Journal of Vascular Surgery Volume 15, Issue 4, Pages 649-656 (April 1992) DOI: 10.1016/0741-5214(92)90010-6 Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 1 Rotation device for lining small caliber cylinders of Gore-Tex (PTFE) prosthesis with ECM and endothelium. The device consists of four axes rotating at a velocity of four rotations/hour, to guarantee even distribution of adhering ECs. PTFE cylinders (6 cm length × 0.6 cm diameter) were used for lining experiments. Bovine corneal cells in DMEM containing 5% FCS, 10% CS, 4% dextran, and 10 ng/ml FGF were seeded at a concentration of 5 × 105 cells/cm2 on segments first coated with fibronectin. Journal of Vascular Surgery 1992 15, 649-656DOI: (10.1016/0741-5214(92)90010-6) Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 2 Proliferation of bovine corneal ECs seeded on tissue culture plastic or PTFE. Bovine ECs were seeded (2.5 × 104 cells/well) on tissue culture wells or PTFE segments (precoated with various substrates). Seven days after seeding, the cells were dissociated with STV and counted. A, Nontreated; B, Fibronectin; C, Collagen; D, Fibronectin + collagen. Journal of Vascular Surgery 1992 15, 649-656DOI: (10.1016/0741-5214(92)90010-6) Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 3 Attachment and proliferation of bovine aortic ECs on Gore-Tex (PTFE) disks coated with different biosubstrates. A, Adult bovine ECs were seeded (2 × 103 cells/well) on untreated PTFE, PTFE coated with ECM, or PTFE that was first coated with fibronectin and then by ECM. After 7 days in culture cells were dissociated with STV and counted. B, Adult bovine ECs seeded (2 × 103 cells/well) on untreated PTFE disks (control) or on PTFE disks coated with fibronectin, fibronectin + ECM, or Matrigel. After 1 day (“attachment”) and 8 days (“proliferation”) in culture, cells were dissociated with STV and counted. No FGF was added during this experiment. Journal of Vascular Surgery 1992 15, 649-656DOI: (10.1016/0741-5214(92)90010-6) Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 4 Scanning electron micrographs of ECM formed on plain PTFE and on PTFE that was first coated with fibronectin. a, ECM secreted on PTFE previously coated with fibronectin. ECM covers the graft in an almost complete and continuous manner. Texture is thick and gaps (holes) in the matrix are few and small. (Original magnification × 400.) b, same as a, but original magnification × 2200. c, ECM secreted directly on plain PTFE. Texture is thinner and many gaps exist of a bigger size than those seen on ECM covering fibronectin-coated PTFE. (Original magnification × 300.) d, same as c, but original magnification × 2000. Journal of Vascular Surgery 1992 15, 649-656DOI: (10.1016/0741-5214(92)90010-6) Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 5 Scanning electron micrographs of corneal and vascular ECs cultured on coated PTFE. A, Bovine corneal ECs seeded on PTFE tubes coated with fibronectin and grown (as described in “Material and Methods”). Seven days later tubes were opened lengthwise and fixed for electron microscopy. (Original magnification × 1000.) B, Bovine aortic ECs seeded on ECM produced by corneal ECs described in A. (Original magnification × 1000.) Journal of Vascular Surgery 1992 15, 649-656DOI: (10.1016/0741-5214(92)90010-6) Copyright © 1992 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions