Identification of RASA1- and NF1-mutated lung cancer cell lines in the CCLE dataset (accessed via the cBioPortal for Cancer Genomics at https://cbioportal.mskcc.org/).

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Identification of RASA1- and NF1-mutated lung cancer cell lines in the CCLE dataset (accessed via the cBioPortal for Cancer Genomics at https://cbioportal.mskcc.org/). Identification of RASA1- and NF1-mutated lung cancer cell lines in the CCLE dataset (accessed via the cBioPortal for Cancer Genomics at https://cbioportal.mskcc.org/). A, Oncoprint (top) of CCLE lung cancer cell lines with alterations in one of the 6 genes indicated (percentages based on total of 181 lines) and table (bottom) of subset with truncating alterations in RASA1 and/or NF1 (copy number and expression data from cBioPortal; expression percentile relative to entire CCLE dataset based on mRNA z-score). B, RASA1 and NF1 protein levels in a panel of 26 human lung cancer cell lines, as determined by Western blotting. Lung cancer cell lines commonly retain RASA1 expression; the exceptions were EPLC272H and RERFLCKJ. C, Western blots of phosho (p)-AKT and pERK levels in HBEC3KT (left), RERFLCKJ (center), and EPLC272H (right) cells following expression of control vector or RASA1. Restoration of RASA1 expression resulted in reduced signaling of MAPK pathway only in RASA1-mutated cells (RERFLCKJ), but not in RASA1/NF1 co-mutated NSCLC lines (EPLC272H). Takuo Hayashi et al. Clin Cancer Res 2018;24:1436-1447 ©2018 by American Association for Cancer Research