Fig. 5. Scanning electron microscopic (SEM) and transmission electron microscopic (TEM) findings of cultured ARNE cells at 14 days after confluence. SEM.

Slides:

Advertisements

Similar presentations

Microscopes. Simple Light Microscopes Contains one lens Uses light Used by Anton van Leeuwenhoek in the 1600s.

Advertisements

Microscopes. Purpose of scopes Magnification – make objects appear larger Resolution – keep the image clear.

Tools of a Biologist. Microscopes: number one tool of a biologist Micro=Scope= – Small-to view A microscope is an instrument used to view very small objects.

Transmission Electron Microscope

Microscopes Section 3-1.

Microscopes Section 2.2. History and use of the microscope

Microscopes are used to increase the magnification and resolving power of the unaided eye MICROSCOPES.

Unit 4: Microscopes, Structure and Function of Cells Microscopes -The Cell Theory HONORS BIOLOGY Monkemeier 2014.

Tools of a Biologist.

Chapter 3 Section 1 Microscopes. Units of Measure  Metric system of measurement  International System of Measurement SI  Base Unit is the Meter (m)

MICROSCOPES A. COMPOUND LIGHT MICROSCOPES. FEATURES Magnification 10X to 400X Formula for determining total magnification – objective lens X ocular.

3.1 Cell Theory KEY CONCEPT Cells are the Basic unit of life.

What is the most dangerous thing in a science lab?

Cell Discovery and Theory History of the Cell Theory  In 1665, Robert Hooke invented the first simple microscope.  He looked at a cork.

Introduction to the Microscope. Types of Microscopes The models found in most schools, use compound lenses to magnify objects. The lenses bend or refract.

Light Microscopes Light Microscopes Compound Light Microscope Focuses with light and lenses. Can be used with living specimens Light weight and portable.

1.5 Technological Advances of the Microscope (Text pages 17 and 18)

Microscope Technology

2. Plant vs Animal Cells Describe the differences between plant and animal cells Identify organelles under the electron microscope.

Transmission Electron Microscope Scanning Electron Microscope Light Microscope.

Chapter 6 Biology – Campbell • Reece

I. Why Study Cells? A. Body is made up of cells: 1. RBC 2. Nerve Cells 3. Skin Cells 4. Muscle Cells.

SC.912.L.14.4 TYPE AND FUNCTION OF VARIOUS MICRSCOPES Compare and Contrast the structure and function of various microscopes.

Microscopes  How can we observe cells?. What technology allows biologists to study microscopic life?  A. Compound Light Microscope 1.Has two lenses.

The Microscope An optical instrument used for viewing very small objects invisible to the naked eye, typically magnified several hundred times.

Microbiology: why should we care?. There are 10 times more bacteria in your digestive system than there are cells in your entire body.

Tools of Science. Microscopes Magnify, or make the image appear larger than it really is Most important tool of the biologist.

Fig. 3. IL-4, IFN-γ, and IL-17 production from α-GalCer-stimulated iNKT cells following FlaB treatment of PBMC cultures from asthma patients and healthy.

Microscopes How can we observe cells?.

Fig. 5. Effects of siRNA against TLR2 and TLR5 on the expression of thymic stromal lymphopoietin (TSLP) from nasal polyp fibroblasts. Fibroblasts were.

Fig. 2. IgE Immunoblotting analysis with pooled sera of egg-allergic patients. (A) shows an immunoblot result using native serum. (B) is a immunoblot result.

Essay writing The importance of advancing techniques in microscopy on identifying the structure and function of cells and their constituents for.

Fig. 2. Effect of PGE2 on VEGF production in NPDFs as determined by RT-PCR (A, C) and ELISA (B, D). (A, B) RT-PCR and ELISA showed that the relative ratio.

Microscope Light Microscope Electron Microscope.

KEY CONCEPT Technology continually changes the way biologists work.

Do Now How did the invention of the microscope help scientists make advances in Biology?

Striking deposition of toxic eosinophil major basic protein in mucus: Implications for chronic rhinosinusitis Jens U. Ponikau, MD, David A. Sherris,

Notes: Cell Theory.

1. Why did Hook use the term cell? 2. List the 3 parts of cell theory?

Microscopes Section 4.2.

Activation of protease-activated receptor 2 leads to impairment of keratinocyte tight junction integrity Peter Nadeau, BS, Mason Henehan, BS, Anna De.

A C B D 1. Read the information about different sorts of microscope.

Microscopes How can we observe cells? HB p. 31.

Role of TWIK-related potassium channel-1 in chronic rhinosinusitis

Adhesion of Streptococcus pneumoniae to human airway epithelial cells exposed to urban particulate matter Naseem Mushtaq, PhD, Majid Ezzati, PhD, Lucinda.

Receptor-interacting protein kinase 3 controls keratinocyte activation in a necroptosis- independent manner and promotes psoriatic dermatitis in mice

Effect of antiangiogenic TKIs and rMVA–CEA–TRICOM vaccine on tumor compactness, tight junctions, and intratumoral pressure in the MC38-CEA model. Effect.

1-4 Tools and Procedures.

The Microscope Appendix C pp.4-5

Monocyte-derived dendritic cell recruitment and allergic TH2 responses after exposure to diesel particles are CCR2 dependent Sharen Provoost, MSc, Tania.

Fig. 3. (A) Effect of EP receptor agonists on VEGF production by NPDFs

Fig. 3. Reporter assay for the RS-ATL8 reporter cell line against shrimp extracts (A, B) RS-ATL8 cells were sensitized overnight with diluted pooled sera.

Fig. 6. Mucus analysis of lungs from allergic and tolerized mice

Fig. 2. Binding of specific IgE to D20110 and S47W

Microscopes Chapter 7.

Defective epithelial barrier in chronic rhinosinusitis: The regulation of tight junctions by IFN-γ and IL-4 Michael B. Soyka, MD, Paulina Wawrzyniak,

Fig. 4. Levels of serum total IgE and IgE concentrations secreted into supernatants of the PBMCs cultured for 3, 5, and 7 days the AR patients and the.

*P < 0.01 and †P < The experiments were repeated 6 times.

Fig. 2. Scanning electron microscopic (SEM) and Transmission electron microscopic (TEM) findings of cultured NHNE cells at 14 days after confluence. SEM.

An ex vivo model of severe asthma using reconstituted human bronchial epithelium Delphine Gras, PhD, Arnaud Bourdin, MD, PhD, Isabelle Vachier, PhD, Laure.

Supplementary Fig. S5. Protein profiles in the DIA set

Supplementary Fig. S2. Profile plot of the proteomics analysis

Fig. 1. Immunohistochemical staining for TMEM16A and MUC5AC in human sinonasal epithelium: TMEM16A and MUC5AC proteins in human nasal polyp epithelium.

Presentation transcript:

Fig. 5. Scanning electron microscopic (SEM) and transmission electron microscopic (TEM) findings of cultured ARNE cells at 14 days after confluence. SEM findings of cultured ARNE cells show smaller numbers of ciliated cells and higher numbers of secretory cells (A, original magnification ×100) (B, original magnification × 200). TEM findings of loose cell-to-cell tight junctions in nasal epithelium and increased number of columnar secretory cells containing electron-lucent secretory granules (C, original magnification ×1,000) (D, original magnification ×2,000). The mRNA levels of E-cadherin and ZO-1 were measured in ALI culture of NHNE and ARNE cells 14 days after confluence. Real-time PCR showed that both E-cadherin (E) and ZO-1 (F) mRNA level were significantly attenuated in ALI culture of ARNE cells (White dot: NHNE cells, Black dot: ARNE cells). Results are presented here as the mean±SD from eight independent ALI cultures (*P<0.05 compared to mRNA levels in NHNE cells). Fig. 5. Scanning electron microscopic (SEM) and transmission electron microscopic (TEM) findings of cultured ARNE cells at 14 days after confluence. SEM findings of cultured ARNE cells show smaller numbers of ciliated cells and higher numbers of secretory cells (A, original magnification ×100) (B, original magnification × 200). TEM findings of loose cell-to-cell . . . Allergy Asthma Immunol Res. 2016 Jan;8(1):69-78. http://dx.doi.org/10.4168/aair.2016.8.1.69