Effects of W. chinensis extract on androgen activity and growth of prostate cancer cells. Effects of W. chinensis extract on androgen activity and growth.

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Presentation transcript:

Effects of W. chinensis extract on androgen activity and growth of prostate cancer cells. Effects of W. chinensis extract on androgen activity and growth of prostate cancer cells. A, dose-response curves of W. chinensis extract in inhibiting 5α-dihydrotestosterone–induced PSA-luciferase reporter. 103E cells were grown and cotreated with 10 nmol/L 5α-dihydrotestosterone and indicated concentrations of W. chinensis extract for 24 h. PSA-luciferase activities of treated cells were measured to determine androgen activity. Mean ± SD of three independent experiments of three replicates. Lower, PSA expression in LNCaP cells co-treated with 10 nmol/L 5α-dihydrotestosterone and indicated amount of H2O-Sub for 24 h. B, colony-forming growth was analyzed by growing LNCaP and 22Rv1 cells (AR-dependent prostate cancer cells) and PC-3 cells (AR-independent prostate cancer cells) in 24-well multidishes with indicated concentrations of W. chinensis extract for 12 d. Colony-forming growth was quantified, and dose-response curves are presented as percent inhibition with respect to control cells (vehicle treatment) of the same cell line. Mean ± SD of three independent experiments of three replicates. C, three different prostate cancer cell lines (a-c) were treated with extract (H2O-Sub) or formula for 48 h. The cell cycle profiles of cancer cells after herbal treatment compared with control (ethanol treatment) were determined by flow cytometric analysis. a, results in LNCaP of different treatments (top) and magnified view of proportion of sub-G1 cells (bottom). Chin-Hsien Tsai et al. Clin Cancer Res 2009;15:5435-5444 ©2009 by American Association for Cancer Research