Hypoxia upregulates CD137 expression in T lymphocytes undergoing activation. Hypoxia upregulates CD137 expression in T lymphocytes undergoing activation.

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Hypoxia upregulates CD137 expression in T lymphocytes undergoing activation. Hypoxia upregulates CD137 expression in T lymphocytes undergoing activation. A, total splenocytes from C57Bl/6 mice were cultured with latex beads coated with anti-CD3- and anti-CD28–specific mAb at a suboptimal 1:20 bead:cell ratio. Histograms represent CD137 expression in 48-hour cultures analyzed by flow cytometric gating on CD3+CD4+- or CD3+CD8+-activated lymphoblasts. CD137-specific stainings were compared with a control isotype–matched antibody tagged with the same fluorochrome (shaded histogram) and are presented in histograms of the indicated line color depending on whether the culture was conducted under normoxia (21% O2) or hypoxia (1% O2). B and C, splenocytes from OT1 or OT-2 TCR-transgenic mice were cultured for 24 hours in the presence of the corresponding cognate OVA peptides at 1 μg/mL either under normoxia or hypoxia. CD137 expression was measured by flow cytometry at 24 hours of culture gating on CD8+ or CD4+ T cells (left) and by quantitative RT-PCR (right). *, P < 0.05. Asís Palazón et al. Cancer Discovery 2012;2:608-623 ©2012 by American Association for Cancer Research