KIR2DL4 on human mast cells upregulates the invasive activity of HLA-G+ cancer cell lines. KIR2DL4 on human mast cells upregulates the invasive activity.

Slides:

Advertisements

Similar presentations

Volume 36, Issue 6, Pages (June 2015)

Advertisements

Cell Physiol Biochem 2016;40: DOI: /

Volume 139, Issue 3, Pages e8 (September 2010)

Myc‐induced miRNAs attenuate differentiation of c‐Myc‐depleted ES cells on LIF withdrawal. Myc‐induced miRNAs attenuate differentiation of c‐Myc‐depleted.

Cyclooxygenase Regulates Angiogenesis Induced by Colon Cancer Cells

by Norman Nausch, Ioanna E

Prostaglandin E2 is a key factor for CCR7 surface expression and migration of monocyte-derived dendritic cells by Elke Scandella, Ying Men, Silke Gillessen,

The formin DIAPH1 (mDia1) regulates megakaryocyte proplatelet formation by remodeling the actin and microtubule cytoskeletons by Jiajia Pan, Larissa Lordier,

Volume 11, Issue 2, Pages (February 2005)

Myeloma-derived Dickkopf-1 disrupts Wnt-regulated osteoprotegerin and RANKL production by osteoblasts: a potential mechanism underlying osteolytic bone.

Expression and regulation of MD-2s.

Volume 139, Issue 3, Pages e8 (September 2010)

A, RT-qPCR of ROR1 mRNA expression in B-CLL cells and a panel of human and rhesus macaque tissues. A, RT-qPCR of ROR1 mRNA expression in B-CLL cells and.

Volume 115, Issue 2, Pages (August 1998)

Volume 22, Issue 10, Pages (October 2014)

B7-H4 expression correlates with MHC-I expression and improved prognosis in patients with breast cancer. B7-H4 expression correlates with MHC-I expression.

Maturation, Ag presentation capacity, and IL-12 production of DCs

A B C D ID8 vector scr sh cx2 cx3 cx4 cx6 CX3CR1 β-tubulin * ID8

A B C D eIF3e shRNA 205 clones Control shRNA 3 7 eIF3e b-actin

FOXO3a Is Activated in Response to Hypoxic Stress and Inhibits HIF1-Induced Apoptosis via Regulation of CITED2 Walbert J. Bakker, Isaac S. Harris, Tak.

Inhibition of β-catenin by the Smad4/BMP pathway.

Molecular Therapy - Nucleic Acids

Knocking down Wnt3 increases the cells' response to trastuzumab and reduces cells' invasiveness. Knocking down Wnt3 increases the cells' response to trastuzumab.

PD-1 blockade enhances T-cell function.

Knockdown of endogenous WT-hTER by a short hairpin RNA (siRNA) directed specifically against the human WT telomerase RNA template. Knockdown of endogenous.

Ectopic expression of caveolin-1 in ZR75 human breast cancer cells downregulated survivin and decreased cell proliferation. Ectopic expression of caveolin-1.

Effect of PDGF-BB on mesenchymal mesonephric cell migration.

Dox titration and kinetic analyses of CD19CAR expression upon Dox administration and discontinuation. Dox titration and kinetic analyses of CD19CAR expression.

M-MDSC:mast cell cross-talk in the regulation of TNFα production.

KIR-CARs/Dap12 trigger robust antigen-specific cytotoxicity in vitro.

Stimulatory effect induced by docetaxel, gefitinib, and cyclopamine on MMP (A) and (B) cytosolic cytochrome c level. Stimulatory effect induced by docetaxel,

A. A. XPCKD and KIN17KD HeLa cells grow poorly 2 weeks after transfection. Forty-eight hours after transfection, cells were plated at the same density.

Tumor MMP-1 functionally regulates the permeability of endothelial cell barriers and contributes to HEp3-hi/diss transendothelial migration. Tumor MMP-1.

In vitro invasion assays were done in the Chemicon invasion chamber containing extracellular matrix. In vitro invasion assays were done in the Chemicon.

Anti-CD20 CAR mRNA enhances exPBNK in vitro cytolytic activity against CD20+ B-NHL cells and rituximab-resistant cells. exPBNK were electroporated in the.

Fluorescence-activated cell sorting (FACS) and clonogenicity analyses.

Effects of WDL on in vitro invasion and soft agar colony formation by LNCaP cells. Effects of WDL on in vitro invasion and soft agar colony formation by.

Overexpression of miR-335, but not miR-29a, reduces in vitro carcinogenesis. Overexpression of miR-335, but not miR-29a, reduces in vitro carcinogenesis.

Mcl-1 knockdown promotes cleavage of caspase-3 in nonadherent melanoma cells. Mcl-1 knockdown promotes cleavage of caspase-3 in nonadherent melanoma cells.

Β-Cryptoxanthin at a concentration of 10 μmol/L decreases proliferation in HCT116 cells after 6 and 8 days of treatment. β-Cryptoxanthin at a concentration.

FOXO1 inhibits Runx2-induced invasion of prostate cancer cells.

CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection of CXCR4 siRNAs. CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection.

The expression of d-GM3 facilitates melanoma cell migration and invasion by activating p38 MAPK, not ERK. A, cell migration and invasion in d-GM3–positive.

A, RT-PCR expression of matriptase-1 and matriptase-2 in a variety of 24 human cell lines. A, RT-PCR expression of matriptase-1 and matriptase-2 in a variety.

TFAP2A knockdown inhibits cell growth by targeting HIF-1α signaling.

IL8 and TNFα are required for neutrophil-induced UMSCC47 increase in invasion. IL8 and TNFα are required for neutrophil-induced UMSCC47 increase in invasion.

Uptake of TEX by DCs in vivo.

Canonical but not adaptive NK-cell function is suppressed by Tregs.

Secretion of TNF-α from macrophages and monocytic cells were reduced by the deletion of Nrdc. Secretion of TNF-α from macrophages and monocytic cells were.

Per1 inhibits growth and induces apoptosis in prostate cancer cell lines. Per1 inhibits growth and induces apoptosis in prostate cancer cell lines. LNCaP,

Combination of miR-520d-3p and EphA2 siRNA treatment shows enhanced EphA2 inhibition and antitumor efficiency in vitro. Combination of miR-520d-3p and.

Effect of nivolumab or ipilimumab on immune responses to vaccination in cynomolgus monkeys. Effect of nivolumab or ipilimumab on immune responses to vaccination.

Neutrophils increase UMSCC47 invasion.

Pirh2 represses p73-dependent transactivation.

Effects of HDAC2 inactivation on the invasive potential of human gastric cancer cells. Effects of HDAC2 inactivation on the invasive potential of human.

Platinum-based chemotherapy increased TXNIP expression, and overexpression of TXNIP enhanced the effectiveness of this therapy. Platinum-based chemotherapy.

FBXL19-AS1 knockdown inhibits proliferation, migration and invasion, and reduces the expression levels of angiogenesis related proteins in lung cancer.

Adhesion to fibronectin protects Mcl-1 knockdown cells from apoptosis.

Role of NO and IFNγ in mast cell–dependent MDSC-suppressive activities

Transcriptional activity and growth of mutant ER in a breast cancer cell line. Transcriptional activity and growth of mutant ER in a breast cancer cell.

VEGF165 stimulates migration of HMVECs

Effects of ASGR2 knockdown on the phenotypes of MKN1 cells.

αvβ6 functional assays in the β cell line using blocking antibodies.

Hypoxia upregulates CD137 expression in T lymphocytes undergoing activation. Hypoxia upregulates CD137 expression in T lymphocytes undergoing activation.

Increased tumorigenic activities of TIM-3–expressing RCC cells.

MPD-L1–transfected 300 cells do not bind to mB7-1–transfected 300 cells. mPD-L1–transfected 300 cells do not bind to mB7-1–transfected 300 cells. Cell-to-cell.

Increased invasion does not require direct contact between neutrophils and UMSCC47. Increased invasion does not require direct contact between neutrophils.

MSCs elicit EMT, invasion of carcinoma cells, and increased tumor initiation of xenografts. MSCs elicit EMT, invasion of carcinoma cells, and increased.

miR-181a knockdown inhibits VEGF and MMP1 secretion in vitro.

Silencing E4BP4 abrogates the promoting effect of SMAD3 knockdown on IFNγ production in NK-92 cells. Silencing E4BP4 abrogates the promoting effect of.

Presentation transcript:

KIR2DL4 on human mast cells upregulates the invasive activity of HLA-G+ cancer cell lines. KIR2DL4 on human mast cells upregulates the invasive activity of HLA-G+ cancer cell lines. A, KIR2DL4-knockdown LAD2 cells were prepared using KIR2DL4-targeting shRNA lentiviral particles (Santa Cruz Biotechnology) according to the manufacturer's instructions. After selection of the KIR2DL4-knockdown LAD2 cells, we collected mRNA from each cell line and performed RT-PCR and flow cytometry as described in Materials and Methods. B, invasion of HLA-G+ cancer cell lines, MCF-7 and JEG-3, that were cocultured with LAD2 cells through Matrigel. A two-chamber invasion assay was performed using Matrigel-coated Transwell polycarbonate membranes (8-μm pores; BD Biosciences). We added to the upper chamber either 5 × 103 MCF-7 or JEG-3 cells alone, or 5 × 103 MCF-7 or JEG-3 cells together with 5 × 103 LAD2 cells that had been transfected with the control vector, or 5 × 103 MCF-7 or JEG-3 cells together with 5 × 103 KIR2DL4-knockdown LAD2 cells. In some experiments, the Fab fragment of anti-HLA-G (Clone 87G) was added. After 6 hours, cells that had migrated to the lower chamber were fixed by 4% PFA, stained with Diff-Quick stain (Sysmex), and counted using a microscope. Three to six independent experiments were performed. *, P < 0.05, compared with the value of MCF-7 or JEG3 cells alone. **, P < 0.05, compared with the value of MCF-7 or JEG3 cells together with mock LAD2 cells. C, MMP-9 production by LAD2 cells only (left), by HLA-G+ cancer cells, MCF-7 (middle), and JEG-3 (right), that were cocultured with LAD2 cells. 5 × 103 mock or KIR2DL4-knockdown LAD2 cells with or without anti-KIR2DL4 Abs (Clone 181703), MCF-7 or JEG-3 cells (grown to confluence) alone, MCF-7 or JEG-3 cells together with 5 × 103 LAD2 cells that had been transfected with the control vector, or MCF-7 or JEG-3 cells together with 5 × 103 KIR2DL4-knockdown LAD2 cells were cultured in a 24-well dish for 6 hours. The resultant culture supernatants were collected and used for the MMP-9 ELISA according to the manufacturer's protocol. *, P < 0.05, compared with the value of MCF-7 or JEG3 cells alone. **, P < 0.05, compared with the value of MCF-7 or JEG3 cells together with mock LAD2 cells. Chiyuki Ueshima et al. Cancer Immunol Res 2015;3:871-880 ©2015 by American Association for Cancer Research