Typical pattern of the degranulation of NK cells from a healthy donor (upper row) and a patient with FRH in recurrence (middle row) and in remission (lower.

Slides:

Advertisements

Similar presentations

Flow cytometry-based diagnosis of primary immunodeficiency diseases

Advertisements

Representative flow cytometry dot plots from a healthy individual

Cytokine-induced rapid ICAM-1 expression on human ASM promotes adhesion of activated T lymphocytes. Cytokine-induced rapid ICAM-1 expression on human ASM.

Melanoma donor (MD) NK cells are functionally impaired/exhausted.

Composition of Innate Lymphoid Cell Subsets in the Human Skin: Enrichment of NCR+ ILC3 in Lesional Skin and Blood of Psoriasis Patients Marcel B.M. Teunissen,

CD56+/CD16− Natural Killer cells expressing the inflammatory protease granzyme A are enriched in synovial fluid from patients with osteoarthritis P.

Human CD1c+ dendritic cells secrete high levels of IL-12 and potently prime cytotoxic T-cell responses by Giulia Nizzoli, Jana Krietsch, Anja Weick, Svenja.

Penicillium marneffei infection and impaired IFN-γ immunity in humans with autosomal- dominant gain-of-phosphorylation STAT1 mutations Pamela P.W. Lee,

Volume 137, Issue 3, Pages e7 (September 2009)

CD300a is expressed on human B cells, modulates BCR-mediated signaling, and its expression is down-regulated in HIV infection by Rodolfo Silva, Susan Moir,

IL-2 consumption by highly activated CD8 T cells induces regulatory T-cell dysfunction in patients with hemophagocytic lymphohistiocytosis Stéphanie.

IRF4 is required for anabolic induction of CD4+ T cells.

Comparison of primary human cytotoxic T-cell and natural killer cell responses reveal similar molecular requirements for lytic granule exocytosis but differences.

CD56+/CD16− Natural Killer cells expressing the inflammatory protease granzyme A are enriched in synovial fluid from patients with osteoarthritis P.

Volume 138, Issue 4, Pages (April 2010)

Targeting PKC in Human T Cells Using Sotrastaurin (AEB071) Preserves Regulatory T Cells and Prevents IL-17 Production Xuehui He, Hans J.P.M. Koenen,

Human lung natural killer cells are predominantly comprised of highly differentiated hypofunctional CD69−CD56dim cells Nicole Marquardt, PhD, Eliisa.

Immunophenotyping of lymphocyte subsets.

DCs induce the proliferation of autologous T cells.

Functional characteristics of neutrophils prior to and following (phorbol myristate acetate) PMA stimulation. Functional characteristics of neutrophils.

Flow cytometric characterization of γδ T cells in our patient demonstrates specific expansion of a homogeneous Vδ2+ T cell population. Flow cytometric.

KIR2DS2 recognizes the HCV peptide LNP.

LRBA protein expression in lymphocytes from a healthy individual and a patient. LRBA protein expression in lymphocytes from a healthy individual and a.

Assessment of CD40L expression and function for diagnosis of X-linked hyper-IgM syndrome (XL-HIGM). Assessment of CD40L expression and function for diagnosis.

Flow cytometry analysis of TNF-β- and IL-10-producing CD33+ cells.

DOCK8 protein expression in lymphocytes from a healthy individual.

Tetramer staining performed to determine frequencies of epitope-specific T cells after clearance of HPV16 infection but prior to isolation of T-cell clones.

Assessment of lymphocyte telomere length in a patient with dyskeratosis congenita. Assessment of lymphocyte telomere length in a patient with dyskeratosis.

Abnormal Natural Killer Cell Function in Systemic Sclerosis: Altered Cytokine Production and Defective Killing Activity Mayuka Horikawa, Minoru Hasegawa,

ISS forming highly aggregated structures have decreased capability to induce maturation of human PDCs. Purified PDCs (105 cells) were stimulated with ISS.

Differential expression of TRM markers by donor- and recipient-derived T cells with time. Differential expression of TRM markers by donor- and recipient-derived.

A novel hypomorphic mutation in STIM1 results in a late-onset immunodeficiency Heidi Schaballie, MD, Rémy Rodriguez, MS, Emmanuel Martin, PhD, Leen Moens,

Foxp3+ Regulatory T Cells of Psoriasis Patients Easily Differentiate into IL-17A- Producing Cells and Are Found in Lesional Skin H. Jorn Bovenschen, Peter.

Reproducibility of LPA results for cryopreserved PBMC after variable periods of storage in liquid nitrogen. Reproducibility of LPA results for cryopreserved.

Viability and viable cell recovery of cryopreserved PBMC after variable periods of storage in liquid nitrogen. Viability and viable cell recovery of cryopreserved.

MK-8628 suppresses T lymphocyte proliferation.

CD151 has little effect on diffusion coefficients.

c-Rel expression in donor T cells is increased after allo-HSCT.

Intracellular TNF-α and IL-4 expression in gated CD33+ monocytes by three-color flow cytometry. Intracellular TNF-α and IL-4 expression in gated CD33+

Anti-CD20 CAR mRNA enhances exPBNK in vitro cytolytic activity against CD20+ B-NHL cells and rituximab-resistant cells. exPBNK were electroporated in the.

pDCs from the melanoma environment responded to TLR-L stimulation.

Immunization regimens that include a GLA-SE-formulated protein vaccine generate memory CD4 T cells. Immunization regimens that include a GLA-SE-formulated.

PD-1 inhibition stimulates the proliferation and cytokine secretion of exhausted/senescent CD8+ T cells in vitro. PD-1 inhibition stimulates the proliferation.

Scattergrams showing IgG- and IgA-specific OD490 values measured with the Campylobacter-specific P18/P39 ELISA in sera from healthy controls and from GBS.

NK activity in healthy donors (n=51) and patients with FRH upon recurrence (n=28) and remission (n=23). NK activity in healthy donors (n=51) and patients.

Flow cytometry analysis of intracellular cytokine expression in control PBMC (left set of panels) and following either PMA-ionomycin activation (middle.

Dilution of anti-C6 monoclonal antibody shows the broader linear range of the Multiplex assay in comparison to standard ELISA. Shown is the dilution of.

ALT-803 stimulates proliferation and activation of human NK cells and T cells in vitro. ALT-803 stimulates proliferation and activation of human NK cells.

Reproducibility of CD4+ and CD8+ T-cell enumeration with cryopreserved PBMC after variable periods of storage in liquid nitrogen. Reproducibility of CD4+

Reproducibility of the enumeration of the percentages of CD4+ CD45RA+ CD95−, CD4+ CD38+, and CD8+ CD38+ in cryopreserved PBMC after variable periods of.

Canonical but not adaptive NK-cell function is suppressed by Tregs.

PAP- and PA2024-specific CTLs at week 0 (prior to sipuleucel-T infusion) and at weeks 6 and 26 post–sipuleucel-T treatment. PAP- and PA2024-specific CTLs.

Effect of nivolumab or ipilimumab on immune responses to vaccination in cynomolgus monkeys. Effect of nivolumab or ipilimumab on immune responses to vaccination.

PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate distinct mechanisms of regulation. PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate.

B- and T-cell activity induced by immunization.

Trametinib and combination promote moDC maturation and decrease moDC viability. Trametinib and combination promote moDC maturation and decrease moDC viability.

Mice with a B cell–specific loss of Ets1 have reduced marginal zone B cells and increased ASCs. (A) Flow cytometry analysis of CD21 versus CD23 in gated.

Intact IL-12 signaling is necessary for the generation of human natural killer cells with enhanced effector function after restimulation Venkateswara.

Group-averaged IL-10 levels (in pg/ml) from the Luminex assay for control, CFS with FM, and CFS without FM groups. Group-averaged IL-10 levels (in pg/ml)

Flow cytometric gating strategy used to identify CD69 surface expression on CD4 (left paired columns) and CD8 (right paired columns) T lymphocytes. Flow.

Diethylamine-based measles avidity assay.

Activation status of NK cells is associated with therapeutic effects of TKIs. PBMCs from CML patients were cocultured with HLA class I–deficient K562 cells.

Fig. 5 IL-5–mediated signaling is critical for the development of CD1dintCD5+ Breg precursor cells and IL-10+ Breg cells. IL-5–mediated signaling is critical.

MDSC population in patient peripheral blood.

GK1 enhances the secretion of proinflammatory cytokines resulting from cognate interactions between activated DCs and T cells. GK1 enhances the secretion.

E2 induces IL-17–producing γδ+ T cells in the FGT

Time to culling for all reasons among J5 vaccinates and controls

Lack of innate immune cell and receptor triggering by Adjuplex.

Surface CD4 and CD14 and intracellular TNF-α expression in PBMCs

Surface expression of chemokine receptors on M

Presentation transcript:

Typical pattern of the degranulation of NK cells from a healthy donor (upper row) and a patient with FRH in recurrence (middle row) and in remission (lower row) without stimulation and in response to K562 cells, phorbol 12-myristate 13-acetate plus ionomycin (PMA+ION), and phorbol 12-myristate 13-acetate plus ionomycin plus cytochalasin B (PMA+ION+CB). Typical pattern of the degranulation of NK cells from a healthy donor (upper row) and a patient with FRH in recurrence (middle row) and in remission (lower row) without stimulation and in response to K562 cells, phorbol 12-myristate 13-acetate plus ionomycin (PMA+ION), and phorbol 12-myristate 13-acetate plus ionomycin plus cytochalasin B (PMA+ION+CB). NK cell degranulation assay was performed as described in Materials and Methods, and the results were assessed by flow cytometry. Shown are the results for gated CD3− CD56+ NK cells. The numerators indicate percentages of NK cells that have externalized CD107a during the 4-h incubation period, and the denominators indicate the mean fluorescence intensity (MFI) of CD107a on CD107a+ NK cells (in arbitrary units). Vladimir V. Murugin et al. Clin. Vaccine Immunol. 2011; doi:10.1128/CVI.05084-11