Influence of minocycline on TGF-β1 expression and TGF-β1–induced NF-кB activation in ovarian cancer cells. Influence of minocycline on TGF-β1 expression.

Slides:

Advertisements

Similar presentations

Molecular Therapy - Nucleic Acids

Advertisements

BET inhibition and depletion repress the expression of BRCA1 and RAD51

Recombinant OPCML protein effect in vitro.

a b MCF-7 TR2 MCF-7 TR2 (Fold change) MTT Assay , (Fold change)

Proline supplementation during P5CS protein knockdown suppressed GCN2 activation. Proline supplementation during P5CS protein knockdown suppressed GCN2.

Everolimus Inhibits SMC Proliferation Through Transcriptional Repression of Telomerase (A) Cell proliferation assay in primary murine vascular smooth muscle.

Interfering with HMGA2 expression blocks transformation in metastatic lung cancer cells. Interfering with HMGA2 expression blocks transformation in metastatic.

ER stress response and susceptibility to apoptosis are regulated by TFEB and TFE3 ER stress response and susceptibility to apoptosis are regulated by TFEB.

HPV‐E7 targets RB for induction of ceramide‐dependent mitophagy

Effect of expression of constitutively active CAMKK2 on AMPK activation and the cell cycle in G361 cells. Effect of expression of constitutively active.

Treatment with MI-773 causes changes in p53 status.

HIPK2 decreases the stability of Notch1-IC through proteasome-dependent degradation. HIPK2 decreases the stability of Notch1-IC through proteasome-dependent.

High-affinity binding to the hypervariable region of the β1 I-like domain controls signaling to fibronectin fibrillogenesis. High-affinity binding to the.

Knocking down Wnt3 increases the cells' response to trastuzumab and reduces cells' invasiveness. Knocking down Wnt3 increases the cells' response to trastuzumab.

SIRT1 is downregulated in human gastric cancer (GC).

High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate.

Allopurinol up-regulates DR5 expression in PC-3 and DU145 cells.

Fig. 2. Acetylation stiffens primary cilia.

Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased survivin expression. Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased.

(A) qRT-PCR-analysis of P450 aromatase mRNA in brains from control and d-Asp-treated frogs. (A) qRT-PCR-analysis of P450 aromatase mRNA in brains from.

The effects of the FASN blocker C75 on cell growth and survival (A, E, and F), on expression/phosphorylation of PI3K and MAPK signaling proteins and on.

CLIC1 promotes tumor cell spreading through effects on MLCK

Variation in dUTPase expression in cell lines.

CDCP1 is required for invadopodia formation and ECM degradation by human breast cancer cells. CDCP1 is required for invadopodia formation and ECM degradation.

Silencing hOGG1 triggers caspase-3 and caspase-7 activation in response to H2O2 in GM00637 cells. Silencing hOGG1 triggers caspase-3 and caspase-7 activation.

The role of p53 in H2O2-mediated cell death of hOGG1-deficient H1299 lung carcinoma p53 null cells. The role of p53 in H2O2-mediated cell death of hOGG1-deficient.

Activation of CHOP and DR5 by DIM-C-pPhtBu.

BME treatment increases granzyme B expression in NK 3.3 cells.

Inhibitory effects of nobiletin and resveratrol on LPS/IFN-γ-induced NO generation in RAW cells. Inhibitory effects of nobiletin and resveratrol.

A, GP and GR epithelial tumor cells are equally responsive to gefitinib. A, GP and GR epithelial tumor cells are equally responsive to gefitinib. Equal.

Depletion of HDAC2 sensitizes cells to epirubicin-induced apoptosis.

RhoH reconstitution in HCL represses CD11c promoter activity.

CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection of CXCR4 siRNAs. CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection.

Effects of visfatin on the cell proliferation and phosphorylation of ERK, Akt, and GSK-3β proteins in HCC cells. Effects of visfatin on the cell proliferation.

Fig. 8 C9orf72 knockdown results in an increase in autophagic flux.

Effect of G9a on the expression of GSH synthesis enzymes.

Overexpression of L1 in CRC cells induces NF-κB activation and suppression of p65 expression blocks the capacity to confer liver metastasis. Overexpression.

Curcumin suppresses the expression of antiapoptotic proteins in multiple myeloma cells. Curcumin suppresses the expression of antiapoptotic proteins in.

Effect of SFN on the protein expression of Nrf2, HO-1, and NQO1 in JB6-shMock and JB6-shNrf2 cells. Effect of SFN on the protein expression of Nrf2, HO-1,

Biological effects of BRAF silencing: growth curves of A375 (A) and ARO (B) cell lines in the absence or presence of doxycycline. Biological effects of.

RT-PCR and Western blot analysis of cIAP2 mRNA and protein expression in ovarian cancer cells. RT-PCR and Western blot analysis of cIAP2 mRNA and protein.

CLIC1 is upregulated in invadopodia of fibrin-embedded tumor and endothelial cells. CLIC1 is upregulated in invadopodia of fibrin-embedded tumor and endothelial.

Induction of DNA strand breaks by artesunate.

PKCζ is tyrosine phosphorylated by EGF and contributes to EGF-induced activation of ERK in Mef cells. PKCζ is tyrosine phosphorylated by EGF and contributes.

Pirh2 represses p73-dependent transactivation.

Effects of HDAC2 inactivation on the invasive potential of human gastric cancer cells. Effects of HDAC2 inactivation on the invasive potential of human.

The effects of HDAC2 knockdown on cell-cycle proteins.

1 OPCML negatively regulates a specific repertoire of RTKs

TBK1 signaling also promotes basal autophagy in 8988T cells.

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

AS1411 alters subcellular distribution of PRMT5 in a time-dependent, dose-dependent, and nucleolin-dependent manner. AS1411 alters subcellular distribution.

A, cellular uptake of AS1411 in MCF-7 and MCF-10A cells.

SAHA blocks IR-induced increase of RAD51 protein in MM cells.

Effect of bevacizumab on the proliferation of A2780 cells.

Levels and activity of c-myc are increased in a series of stably transfected DAOY and UW228 medulloblastoma cell lines. Levels and activity of c-myc are.

TGFβ1/Smad3 signaling suppresses IFNγ production via an E4BP4-dependent mechanism in NK-92 cells. TGFβ1/Smad3 signaling suppresses IFNγ production via.

Interaction of NHEJ and HR pathways in PCO cultures.

Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization in 231BR3 cells. Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization.

ABT-888 sensitizes multiple ovarian cancer cell lines but not normal cells to FdUrd. ABT-888 sensitizes multiple ovarian cancer cell lines but not normal.

PARP1 suppresses the transcription of PD-L1 in cancer cells.

RA-9 induces G2–M cell-cycle arrest and caspase-mediated apoptosis in ovarian cancer cells. RA-9 induces G2–M cell-cycle arrest and caspase-mediated apoptosis.

Effect of dexamethasone on PCDGF/GP88 mRNA and protein expressions and effect of PCDGF/GP88 on dexamethasone-induced cell death. Effect of dexamethasone.

EGF induces HPSE nucleolar localization in human BMBC cells.

Autophagy mediates postslippage cell fate.

Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells. Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells.

Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.

Hoxa9 is partly required for Twist1-induced migration, invasion, anoikis resistance, and soft agar tumorigenicity of Myc-CaP cells. Hoxa9 is partly required.

Effect of 6-SHO on mouse prostate cancer HMVP2 cells.

D-GM3 promotes uPAR clustering on the cell surface and activates p38 MAPK. A, uPAR expression in cells was either knocked down by treatment with 4 independent.

Time course of NMT knockdown.

Presentation transcript:

Influence of minocycline on TGF-β1 expression and TGF-β1–induced NF-кB activation in ovarian cancer cells. Influence of minocycline on TGF-β1 expression and TGF-β1–induced NF-кB activation in ovarian cancer cells. A, confocal immunocytochemistry of TGF-β1 (green) in OVCAR-3 and SKOV-3 cells treated with minocycline in comparison with control cells. The nuclei are counterstained with propidium iodide (PI; red). Images were obtained at × 60 magnification. The scale bars represent 100 μm. B, the expression levels of TGF-β1 in cells treated with minocycline (0–100 μmol/L) as estimated by Western blot analysis. The densitometric values quantified using the Quantity One image program (Bio-Rad) were corrected on the basis of β-actin and were expressed as the percentage of the values corresponding to control cells. Columns, means of 3 independent experiments; bars, SD. **, P < 0.01 versus control cells. C, TGF-β1 expression in cells either untransfected or stably transfected with pCMV6-TGF-β/pCMV6-Neo as assessed by Western blotting. β-actin, loading control. D, NF-кB–dependent luciferase activity of TGF-β1–dominant cells treated with vehicle/minocycline (100 μmol/L) expressed as fold change comparing pCMV6-Neo–transfected cells. Results were standardized relative to protein concentration. Columns, means of 3 independent experiments; bars, SD. **, P < 0.01 and ***, P < 0.001 versus pCMV6-Neo–transfected cells. E, TGF-β1 expression in cells either untransfected or stably transfected with Scrambled shRNA/TGF-β1-shRNA as assessed by Western blotting. β-actin, loading control. F, NF-кB activity of Scrambled shRNA/TGF-β1-shRNA–transfected cells treated with vehicle/minocycline (100 μmol/L) assessed by luciferase reporter assay. Results were standardized relative to protein concentration and expressed as fold change comparing with vehicle-treated cells in each group. Columns, means of 3 independent experiments; bars, SD. **, P < 0.01 and ***, P < 0.001 versus vehicle-treated cells in each group. Parvin Ataie-Kachoie et al. Mol Cancer Res 2013;11:1279-1291 ©2013 by American Association for Cancer Research