Representative images for different populations of detected cells in prostate cancer patients and five rounds of FISH in a CTC. A, Immunofluorescence image.

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Representative images for different populations of detected cells in prostate cancer patients and five rounds of FISH in a CTC. A, Immunofluorescence image for three types of circulating cells. Representative images for different populations of detected cells in prostate cancer patients and five rounds of FISH in a CTC. A, Immunofluorescence image for three types of circulating cells. Top row, one CK+/VIM−/CD45− cell (arrowed) adjacent to one CD45+ lymphocyte; middle row, two CK+/VIM+/CD45− cells (arrowed) adjacent to one CD45+ lymphocyte; bottom row, two CK−/VIM+/CD45− cells (arrowed) adjacent to one CD45+ lymphocyte. B, Five rounds of FISH on one CK−/VIM+/CD45− CTC postimmunostaining. First round of FISH: AR (red) and 6q16 (green). Second round of FISH for ERG rearrangement: RP11-476D17 (red) and RP11-95I21 (green). Third round of FISH: C-MYC (red) and NKX3.1 (green). Fourth round of FISH: RB1 (red) and PTEN (green). Fifth round of FISH: CCND1 (red) and 16q22.1 (green). Arrows, FISH signals. Lei Xu et al. Clin Cancer Res 2017;23:5112-5122 ©2017 by American Association for Cancer Research