Growth with N-acetylglucosamine rapidly raises the environmental pH and releases detectable ammonia. Growth with N-acetylglucosamine rapidly raises the.

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Growth with N-acetylglucosamine rapidly raises the environmental pH and releases detectable ammonia. Growth with N-acetylglucosamine rapidly raises the environmental pH and releases detectable ammonia. (A) Wild-type C. albicans strain SC5314 was assessed for pH neutralization in YNBAG plus 20 mM N-acetylglucosamine or 1% Casamino Acids over 24 h. pH was adjusted to 4 with HCl prior to inoculation. (B) Ammonia released by C. albicans cells during pH neutralization on solid medium containing YNBAG plus 20 mM N-acetylglucosamine or 1% Casamino Acids. Ammonia was detected after 24, 48, and 72 h via Nessler’s reagent. *, P < 0.05. Data are expressed as mean values ± standard deviation (SD) from triplicate experiments. YNBAG medium consists of minimal YNB supplemented with 0.5% glycerol and 0.5% allantoin. Elisa M. Vesely et al. mSphere 2017; doi:10.1128/mSphere.00357-17