Fig. 3 Heterologous TRPV1-mediated uptake of gentamicin is attenuated by extracellular [Ca2+]. Heterologous TRPV1-mediated uptake of gentamicin is attenuated.

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Fig. 3 Heterologous TRPV1-mediated uptake of gentamicin is attenuated by extracellular [Ca2+]. Heterologous TRPV1-mediated uptake of gentamicin is attenuated by extracellular [Ca2+]. (A) KPT2-Trpv1 cells treated with GTTR (5 μg/ml) show reduced fluorescence when coincubated with 2 mM extracellular [Ca2+]. The TRPV1 agonist capsaicin (3 μM) visibly increased GTTR fluorescence over untreated KPT2-Trpv1 cells. Capsaicin-enhanced GTTR uptake is visibly attenuated by 2 mM extracellular [Ca2+]. Scale bar, 20 μm. (B) GTTR fluorescence in KPT2-pBabe cells was significantly less than that in KPT2-Trpv1 cells. Extracellular [Ca2+] (2 mM) diminished GTTR fluorescence in KPT2-Trpv1 cells. Capsaicin significantly increased GTTR fluorescence in KPT2-Trpv1 cells but not in KPT2-pBabe cells. Capsaicin-enhanced GTTR uptake in KPT2-Trpv1 cells is significantly attenuated by 2 mM extracellular [Ca2+], without modulating GTTR levels in KPT2-pBabe cells (P > 0.05; n = 4 per group). ELISA data for cellular levels of GTTR (C) and native gentamicin (D) support imaging data described in (A) and (B) (*P < 0.05; n = 4 per group). Error bars, SD. Meiyan Jiang et al. Sci Adv 2019;5:eaaw1836 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).