LY elicits hyperphosphorylation of Akt.

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LY2780301 elicits hyperphosphorylation of Akt. LY2780301 elicits hyperphosphorylation of Akt. A, RPPA analysis showing expression or phosphorylation of proteins significantly changed in 10A.Vec and 10A.B2 cells, treated with vehicle or LY2780301, under both 2D and 3D culture conditions. B, Western blotting of Cyclin B1 in 10A.Vec and 10A.B2 cells treated with LY2780301 at various doses under 2D or 3D conditions. Quantification of Cyclin B1 band was shown underneath each lane. C and D, Western blotting of p-Akt and pan Akt (C), p-Erk1/2 and Erk1/2 (D) in 10A.Vec, and 10A.B2 cells treated with LY2780301 at various doses under 2D or 3D conditions. E, Western blotting of ErbB2, p-S6, S6, p-Akt, and pan Akt in 10A.Vec and 10A.B2 cells treated with LY2780301, alone or in combination with GDC-0941. F, Immunofluorescent images showing Ki-67, cleaved caspase-3, Laminin-332, and DAPI staining in 10A.B2 acini treated with GDC-0941 or in combination with LY2780301. Xiao Wang et al. Cancer Prev Res 2017;10:641-650 ©2017 by American Association for Cancer Research