Establishment and characterization of primary CAFs from patients with colorectal cancer. Establishment and characterization of primary CAFs from patients.

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Establishment and characterization of primary CAFs from patients with colorectal cancer. Establishment and characterization of primary CAFs from patients with colorectal cancer. Schedule of primary CAF establishment from fresh tissue of primary tumors from patients with colorectal cancer (A). Although at different levels, all CAFs showed α-SMA expression. In the images, examples of a low and a high α-SMA–expressing CAF are given (BI and BII, respectively). The absence of epithelial cells in the established primary cultures was confirmed by PanCK and Vimentin immunostaining. LIM1215 colon cells were used as positive control for PanCK immunostaining (C). Senescence-associated β-galactosidase staining was used to evaluate the degree of senescence of primary CAF cultures (DI showed a negative CAF culture and DII, a positive CAF culture for senescence-associated β-galactosidase staining). Mercedes Herrera et al. Clin Cancer Res 2013;19:5914-5926 ©2013 by American Association for Cancer Research