Detection of protein levels of Cdc25AWT and its mutation derivatives in breast cancer cells with I3C treatment. Detection of protein levels of Cdc25AWT and its mutation derivatives in breast cancer cells with I3C treatment. Cdc25AWT and its derivatives continued to be induced in MDA-MD-231 breast cancer cells with 10 ng/mL Dox while the cells were treated with either 0.1% DMSO alone or with 200 μmol/L I3C for 48 h. The cells were then stained with anti-Cdc25A mAb. Signals were visualized by incubation with fluorescein-conjugated anti-mouse immunoglobulin G antibody, followed by analysis with a fluorescence microscope. The cells maintain normal cell shape (phase-contrast images) and 4′,6-diamidino-2-phenylindole staining also shows normal nuclear shape (data not shown). Results are representative of three independent sets of experiments. A, Cdc25AWT; B, Cdc25AS76A; C, Cdc25AS82A; D, Cdc25AS124A. Yongsheng Wu et al. Cancer Prev Res 2010;3:818-828 ©2010 by American Association for Cancer Research