KRAS-addicted lung cancer cells need αvβ3/galectin-3 to maintain low ROS levels. KRAS-addicted lung cancer cells need αvβ3/galectin-3 to maintain low ROS.

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KRAS-addicted lung cancer cells need αvβ3/galectin-3 to maintain low ROS levels. KRAS-addicted lung cancer cells need αvβ3/galectin-3 to maintain low ROS levels. A, The effect of KRAS knockdown on mitochondrial ROS levels was visualized as fluorescence staining using MitoSOX Red or 8-oxo-dG. MitoSOX Red staining was quantified by flow cytometry (4 hours). Scale bar, 10 μm. B, The effect of oxidative stress (hydrogen peroxide) on viability was compared for cells growing in suspension. C, Ectopic β3 expression protects H727 cells from the effects of oxidative stress. D, By flow cytometry, the β3-high population sorted from PDX, PDX-8, shows lower levels of mitochondrial stress (MitoSOX) compared with the β3-low population. E, The effect of β3 or galectin-3 knockdown on ROS levels was evaluated using flow cytometry (MitoSOX). All data represent mean ± SD from at least three independent experiments. Statistical significance was determined by the Student t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Laetitia Seguin et al. Cancer Discov 2017;7:1464-1479 ©2017 by American Association for Cancer Research