Alexander S. Kim, MD, Taylor A. Doherty, MD, Maya R

Slides:

Advertisements

Similar presentations

Comparison of the allergic and nonallergic CD4+ T-cell responses to the major dog allergen Can f 1 Suvi Parviainen, MSc, Antti Taivainen, MD, PhD, Aino.

Advertisements

Innate lymphoid cells contribute to allergic airway disease exacerbation by obesity Laetitia Everaere, PhD, Saliha Ait-Yahia, PhD, Olivier Molendi-Coste,

Pharmacologic rationale for treating allergic and nonallergic rhinitis

Human group 2 innate lymphoid cells do not express the IL-5 receptor

Margaretha A. Faber, MD, Vito Sabato, MD, Chris H

Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy Yin Yao, MD, Cai-Ling.

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection

Rigid substrate induces esophageal smooth muscle hypertrophy and eosinophilic esophagitis fibrotic gene expression Eugene Tkachenko, PhD, Renee Rawson,

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Ann-Marie M. Schoos, MD, PhD, Jacob D

Immunologic defects in 22q11.2 deletion syndrome

Probiotic supplementation has little effect on peripheral blood regulatory T cells Nicholas P. West, PhD, Peggy L. Horn, PhD, David B. Pyne, PhD, Hilary.

Pentraxin 3 deletion aggravates allergic inflammation through a TH17-dominant phenotype and enhanced CD4 T-cell survival Jyoti Balhara, MSc, Lianyu Shan,

Type 2 innate lymphoid cells in induced sputum from children with severe asthma Prasad Nagakumar, MBBS, Laura Denney, PhD, Louise Fleming, MD, Andrew.

IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,

Neonatal rhinovirus induces mucous metaplasia and airways hyperresponsiveness through IL-25 and type 2 innate lymphoid cells Jun Young Hong, MS, J. Kelley.

Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.

Is 9 more than 2 also in allergic airway inflammation?

Responsiveness to respiratory syncytial virus in neonates is mediated through thymic stromal lymphopoietin and OX40 ligand Junyan Han, PhD, Azzeddine.

Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,

Oroscomucoid like protein 3 (ORMDL3) transgenic mice have reduced levels of sphingolipids including sphingosine-1-phosphate and ceramide Marina Miller,

Children with allergic and nonallergic rhinitis have a similar risk of asthma Bo Lund Krogsgaard Chawes, MD, Klaus Bønnelykke, MD, PhD, Eskil Kreiner-Møller,

Lung T-cell responses to nontypeable Haemophilus influenzae in patients with chronic obstructive pulmonary disease Paul T. King, MD, PhD, Steven Lim,

Severe atopic dermatitis is characterized by selective expansion of circulating TH2/TC2 and TH22/TC22, but not TH17/TC17, cells within the skin-homing.

Natural regulatory T cells in isolated early responders compared with dual responders with allergic asthma Takashi Kinoshita, MD, Adrian Baatjes, MSc,

IL-22–producing “T22” T cells account for upregulated IL-22 in atopic dermatitis despite reduced IL-17–producing TH17 T cells Kristine E. Nograles, MD,

Forkhead box protein 3 demethylation is associated with tolerance induction in peanut- induced intestinal allergy Meiqin Wang, MD, PhD, Ivana V. Yang,

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

Jug r 2–reactive CD4+ T cells have a dominant immune role in walnut allergy Luis Diego Archila, MSc, David Jeong, MD, Mariona Pascal, PhD, Joan Bartra,

Yuichi Teraki, MD, Aika Sakurai, MD, Seiichi Izaki, MD

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

Differential expression of functional chemokine receptors on human blood and lung group 2 innate lymphoid cells Cathryn A. Weston, PhD, Batika M.J. Rana,

Katherine G. MacDonald, BSc, Nicholas A. J

Takao Kobayashi, PhD, Koji Iijima, PhD, Alexander L

Corticosteroid-resistant asthma is associated with classical antimicrobial activation of airway macrophages Elena Goleva, PhD, Pia J. Hauk, MD, Clifton.

Peter M. Wolfgram, MD, David B. Allen, MD

Profiling of human CD4+ T-cell subsets identifies the TH2-specific noncoding RNA GATA3-AS1 Huan Zhang, MD, PhD, Colm E. Nestor, PhD, Shuli Zhao, PhD,

Allergen-enhanced thrombomodulin (blood dendritic cell antigen 3, CD141) expression on dendritic cells is associated with a TH2-skewed immune response

Patients with atopic dermatitis and history of eczema herpeticum elicit herpes simplex virus–specific type 2 immune responses Stephan Traidl, Petra Kienlin,

Β2 integrins rather than β1 integrins mediate Alternaria-induced group 2 innate lymphoid cell trafficking to the lung Maya R. Karta, PhD, Peter S. Rosenthal,

Benjamin T. Prince, MD, Msci, Ashley L. Devonshire, MD, MPH, Kristin A

Chronic cat allergen exposure induces a TH2 cell–dependent IgG4 response related to low sensitization Amedee Renand, PhD, Luis D. Archila, MSc, John.

Role of hyaluronan and hyaluronan-binding proteins in human asthma

Successful rapid rituximab desensitization in an adolescent patient with nephrotic syndrome: Increase in number of Treg cells after desensitization Metin.

Brent E. Palmer, PhD, Douglas G. Mack, PhD, Allison K

Group 2 innate lymphoid cells are recruited to the nasal mucosa in patients with aspirin- exacerbated respiratory disease Jacqueline J. Eastman, MD, Kellen.

Does reduced zona pellucida binding protein 2 (ZPBP2) expression on chromosome 17q21 protect against asthma? Marina Miller, MD, PhD, Christine Vuong,

Yilin Qi, MSc, Darwin J. Operario, PhD, Christopher M

Chen Yao, BS, Sandra M. Zurawski, PhD, Elizabeth S

Myeloid and plasmacytoid dendritic cells in induced sputum after allergen inhalation in subjects with asthma Benny Dua, MSc, Richard M. Watson, BSc,

Toll-like receptor 2–positive and Toll-like receptor 4–positive cells in adenoids of children exposed to passive smoking Antonio Ricci, MD, M. Antonietta.

A cluster-randomized trial shows telephone peer coaching for parents reduces children's asthma morbidity Jane M. Garbutt, MB, ChB, Yan Yan, MD, PhD,

Autophagy: Nobel Prize 2016 and allergy and asthma research

IL-2– and CD25-dependent immunoregulatory mechanisms in the homeostasis of T-cell subsets Sven Létourneau, DPhil, Carsten Krieg, PhD, Giuseppe Pantaleo,

Lei Wang, MD, PhD, Jesse P. Joad, MD, Caiyun Zhong, MD, PhD, Kent E

Changes in regulatory B-cell levels in bone marrow, blood, and sputum of patients with asthma following inhaled allergen challenge John-Paul Oliveria,

Matthew J. Loza, PhD, Susan Foster, PhD, Stephen P

Effect of aging on sputum inflammation and asthma control

Allergen-specific immunotherapy modulates the balance of circulating Tfh and Tfr cells Véronique Schulten, PhD, Victoria Tripple, BSc, Grégory Seumois,

Anti–IL-5 therapy reduces mast cell and IL-9 cell numbers in pediatric patients with eosinophilic esophagitis Iris M. Otani, MD, Arjun A. Anilkumar,

Wasp venom immunotherapy expands a subpopulation of CD4+CD25+ forkhead box protein 3–positive regulatory T cells expressing the T-cell receptor Vβ2 and.

Bronchoalveolar lavage invariant natural killer T cells are not increased in asthma Kugathasan Mutalithas, MRCP, Joanne Croudace, PhD, Cristina Guillen,

IL-9 and c-Kit+ mast cells in allergic rhinitis during seasonal allergen exposure: Effect of immunotherapy Kayhan T. Nouri-Aria, PhD, FRCPath, Charles.

Macrolide antibiotics and asthma treatment

Taylor A. Doherty, MD, Rachel Baum, BS, Robert O

Natural history of cow’s milk allergy

Perturbations of natural killer cell regulatory functions in respiratory allergic diseases Francesca Scordamaglia, MD, Mirna Balsamo, PhD, Antonio Scordamaglia,

Eosinophil progenitor levels are increased in patients with active pediatric eosinophilic esophagitis David W. Morris, MD, Emily M. Stucke, BA, Lisa.

Invariant natural killer T cells from children with versus without food allergy exhibit differential responsiveness to milk-derived sphingomyelin Soma.

Comparison of the allergic and nonallergic CD4+ T-cell responses to the major dog allergen Can f 1 Suvi Parviainen, MSc, Antti Taivainen, MD, PhD, Aino.

Presentation transcript:

Regulatory B cells and T follicular helper cells are reduced in allergic rhinitis Alexander S. Kim, MD, Taylor A. Doherty, MD, Maya R. Karta, PhD, Sudipta Das, PhD, Rachel Baum, BS, Peter Rosenthal, BS, Andrew Beppu, BS, Marina Miller, MD, PhD, Richard Kurten, PhD, David H. Broide, MB, ChB Journal of Allergy and Clinical Immunology Volume 138, Issue 4, Pages 1192-1195.e5 (October 2016) DOI: 10.1016/j.jaci.2016.03.017 Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 FACS gating strategy to detect Breg cells, CD25hi Breg cells, and TFH cells in peripheral blood. A, Circulating Breg cells and TFH-like cells were identified from the circulating lymphocyte population as CD19+CD73−CD25+CD71+ and CD4+PD-1+CXCR5+, respectively, using the gating strategy shown. B, Example of FACS of circulating Breg cells from a nonallergic individual (left) and an allergic individual (right) including isotype controls was used to quantitate their total CD25+ and CD25hi subsets. FSC-A, Forward-scatter area; SSC-A, side-scatter area. Journal of Allergy and Clinical Immunology 2016 138, 1192-1195.e5DOI: (10.1016/j.jaci.2016.03.017) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 FACS quantitation of Breg cells, CD25hi Breg cells, and TFH-like cells in subjects with allergic rhinitis and controls. A, Peripheral blood total CD25+ (left) and CD25hi (right) Breg cells from individuals with allergic rhinitis (n = 10) and nonallergic individuals (n = 7) were quantitated as percentages of CD19+ cells. B, Circulating CD4+PD-1+CXCR5+ TFH-like cells from individuals with allergic rhinitis (n = 7) and nonallergic individuals (n = 5) were quantitated as percentages of CD4+ T cells. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology 2016 138, 1192-1195.e5DOI: (10.1016/j.jaci.2016.03.017) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 FACS gating strategy to detect Breg cells and CD25hi Breg cells in human lung lymph nodes. A, Human lung lymph node Breg cells were identified from the lung lymph node lymphocyte population as CD19+CD73−CD25+CD71+ using the gating strategy shown. B, Human lung lymph node Breg cells from an individual with asthma were analyzed using isotype controls to analyze their total CD25+ and CD25hi subsets. Journal of Allergy and Clinical Immunology 2016 138, 1192-1195.e5DOI: (10.1016/j.jaci.2016.03.017) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 FACS gating strategy to detect TFH-like cells in human lung lymph nodes. TFH-like cells from a lung lymph node from an individual with asthma were identified from the lung lymph node lymphocyte population as CD4+CXCR5+PD-1+ using the gating strategy shown. Journal of Allergy and Clinical Immunology 2016 138, 1192-1195.e5DOI: (10.1016/j.jaci.2016.03.017) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Cytokine production by Breg cells and TFH cells. A, IL-10 protein levels measured by ELISA in supernatants of sorted Breg cells from a nonallergic subject. B, IL-21 mRNA expression quantitated by quantitative PCR in sorted TFH cells from a nonallergic subject. Journal of Allergy and Clinical Immunology 2016 138, 1192-1195.e5DOI: (10.1016/j.jaci.2016.03.017) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions