Interaction of cancer spheroids with mesothelium prompts mesothelial cell clearance. Interaction of cancer spheroids with mesothelium prompts mesothelial.

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Interaction of cancer spheroids with mesothelium prompts mesothelial cell clearance. Interaction of cancer spheroids with mesothelium prompts mesothelial cell clearance. A, ovarian cancer spheroids (OVCA433) labeled with CMTCX cell tracker dye (red) were pipetted on top of a confluent monolayer of GFP-labeled primary TERT-immortalized human lung mesothelial cells (green) and incubated for 60 minutes. The dynamics of these 2 cell populations were followed in parallel for 10 hours. Images show a time course of mesothelial clearance at 0, 7, and 10 hours. Scale bar, 100 μm. B, quantification of mesothelial clearance from experiment shown in A. AU, arbitrary units. C, representative images from a mesothelial clearance assay using a primary tumor cluster isolated from the ascites fluid of an ovarian cancer patient. The sample was labeled and assayed as in A. Scale bar, 50 μm. D, time-lapse images of multiple Z sections (side view) of OVCA433 CMTCX-labeled spheroids inducing clearance of GFP-labeled lung mesothelial cells. Marcin P. Iwanicki et al. Cancer Discov 2011;1:144-157 ©2011 by American Association for Cancer Research