In silico coverage across fungal phyla.

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In silico coverage across fungal phyla. In silico coverage across fungal phyla. Predicted taxonomic coverage was assessed using PrimerProspector. (A) The forward literature fungal primers ITS1 (L), ITS1-F (L), and ITS5 (L) had significantly lower overall taxonomic coverage than the (B) newly created forward primers ITS1-27F (N), ITS1-30F (N), ITS1-34F (N), and ITS1-48F (N). The custom-designed reverse primer ITS1-217R (N) and the published reverse primer ITS2 (L) both demonstrated high predicted taxonomic coverage across the phyla. Mykhaylo Usyk et al. mSphere 2017; doi:10.1128/mSphere.00488-17