Claire E. Beyer, B. Sc. , Bettina Kayler, B. M. Sc

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Supersensitive fluorescent semen analysis: validation on azoospermic and oligozoospermic samples Claire E. Beyer, B.Sc., Bettina Kayler, B.M.Sc., Elissa Osborne, Ph.D., Robert McLachlan, M.D., Tiki Osianlis, Ph.D. Fertility and Sterility Volume 98, Issue 4, Pages 843-848.e1 (October 2012) DOI: 10.1016/j.fertnstert.2012.06.036 Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Under fluorescence, two small bright nuclei and one larger nucleus are visible. (B) Using phase-contrast microscopy, the two small bright nuclei have distinguishable sperm tails whereas the larger nucleus does not. A high volume of debris is observed using phase-contrast microscopy. This debris is not observed under fluorescence because not all debris absorbs the fluorescent Hoechst stain. Fertility and Sterility 2012 98, 843-848.e1DOI: (10.1016/j.fertnstert.2012.06.036) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions