Perturbations of natural killer cell regulatory functions in respiratory allergic diseases Francesca Scordamaglia, MD, Mirna Balsamo, PhD, Antonio Scordamaglia,

Slides:

Advertisements

Similar presentations

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy

Advertisements

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy Erik Wambre, PhD, Jonathan.

Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease Merja T. Augustin, MD, Jorma Kokkonen, MD,

Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease Merja T. Augustin, MD, Jorma Kokkonen, MD,

The loss of IgM memory B cells correlates with clinical disease in common variable immunodeficiency Rita Carsetti, MD, Maria Manuela Rosado, PhD, Simona.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut Claudia.

Expression of IL-9 receptor α chain on human germinal center B cells modulates IgE secretion Lama M. Fawaz, PhD, Ehssan Sharif-Askari, PhD, Oumnia Hajoui,

Defective natural killer–cell cytotoxic activity in NFKB2-mutated CVID-like disease Vassilios Lougaris, MD, Giovanna Tabellini, PhD, Massimiliano Vitali,

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites

Ambient particulate matter directs nonclassic dendritic cell activation and a mixed TH1/TH2-like cytokine response by naive CD4+ T cells Marc A. Williams,

Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response Miriam Wittmann, MD, Mareike Alter, Tanja.

B-cell reconstitution after lentiviral vector–mediated gene therapy in patients with Wiskott-Aldrich syndrome Maria Carmina Castiello, PhD, Samantha.

Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis Eva Gros, MSc, Susanne Petzold,

Ann-Marie M. Schoos, MD, PhD, Jacob D

Human B cells promote T-cell plasticity to optimize antibody response by inducing coexpression of TH1/TFH signatures Jelle de Wit, PhD, Tineke Jorritsma,

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut Claudia.

Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,

Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,

Penicillium marneffei infection and impaired IFN-γ immunity in humans with autosomal- dominant gain-of-phosphorylation STAT1 mutations Pamela P.W. Lee,

Anti–IL-5 (mepolizumab) therapy reduces eosinophil activation ex vivo and increases IL- 5 and IL-5 receptor levels Miguel L. Stein, MD, Joyce M. Villanueva,

Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.

Identification of a subset of human natural killer cells expressing high levels of programmed death 1: A phenotypic and functional characterization Silvia.

Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.

IL-2 consumption by highly activated CD8 T cells induces regulatory T-cell dysfunction in patients with hemophagocytic lymphohistiocytosis Stéphanie.

Is 9 more than 2 also in allergic airway inflammation?

Differences in innate immune function between allergic and nonallergic children: New insights into immune ontogeny Meri K. Tulic, BSc, PhD, Megan Hodder,

Lung T-cell responses to nontypeable Haemophilus influenzae in patients with chronic obstructive pulmonary disease Paul T. King, MD, PhD, Steven Lim,

Susan L. Prescott, PhD, MD, Paul Noakes, BSc (Hons), PhD, Bonita W. Y

Yuichi Teraki, MD, Aika Sakurai, MD, Seiichi Izaki, MD

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

CD94/NKG2C is a killer effector molecule in patients with Stevens-Johnson syndrome and toxic epidermal necrolysis Esther Morel, PhD, Salvador Escamochero,

Thymic stromal lymphopoietin converts human epidermal Langerhans cells into antigen- presenting cells that induce proallergic T cells Susanne Ebner, PhD,

Targeting Fel d 1 to FcγRI induces a novel variation of the TH2 response in subjects with cat allergy Kathryn E. Hulse, BS, Amanda J. Reefer, MS, Victor.

Targeting allergen to FcγRI reveals a novel TH2 regulatory pathway linked to thymic stromal lymphopoietin receptor Kathryn E. Hulse, PhD, Amanda J. Reefer,

Tolerogenic signaling by pulmonary CD1c+ dendritic cells induces regulatory T cells in patients with chronic obstructive pulmonary disease by IL-27/IL-10/inducible.

Allison K. Martin, BS, Douglas G. Mack, PhD, Michael T

Benjamin T. Prince, MD, Msci, Ashley L. Devonshire, MD, MPH, Kristin A

Chronic cat allergen exposure induces a TH2 cell–dependent IgG4 response related to low sensitization Amedee Renand, PhD, Luis D. Archila, MSc, John.

Impaired natural killer cell functions in patients with signal transducer and activator of transcription 1 (STAT1) gain-of-function mutations Giovanna.

Brent E. Palmer, PhD, Douglas G. Mack, PhD, Allison K

β-Agonist enhances type 2 T-cell survival and accumulation

Thymic stromal lymphopoietin–activated invariant natural killer T cells trigger an innate allergic immune response in atopic dermatitis Wen Hao Wu, PhD,

IL-17 in atopic eczema: Linking allergen-specific adaptive and microbial-triggered innate immune response Kilian Eyerich, MD, Davide Pennino, PhD, Claudia.

Peanut-specific type 1 regulatory T cells induced in vitro from allergic subjects are functionally impaired Laurence Pellerin, PhD, Jennifer Anne Jenks,

Role of B cells in TH cell responses in a mouse model of asthma

Mammalian target of rapamycin inhibition counterbalances the inflammatory status of immune cells in patients with chronic granulomatous disease Aurélie.

Expression of IL-9 receptor α chain on human germinal center B cells modulates IgE secretion Lama M. Fawaz, PhD, Ehssan Sharif-Askari, PhD, Oumnia Hajoui,

Toll-like receptor 2–positive and Toll-like receptor 4–positive cells in adenoids of children exposed to passive smoking Antonio Ricci, MD, M. Antonietta.

Demonstration of circulating allergen-specific CD4+CD25highFoxp3+ T-regulatory cells in both nonatopic and atopic individuals Laura Maggi, BSc, Veronica.

Staphylococcal exotoxins are strong inducers of IL-22: A potential role in atopic dermatitis Margarete Niebuhr, MD, Helena Scharonow, MS, Merle Gathmann,

Peanut T-cell epitope discovery: Ara h 1

Cell surface characterization of T lymphocytes and allergen-specific T cell clones: Correlation of CD26 expression with T H1 subsets Martin Willheim,

Grass tablet sublingual immunotherapy downregulates the TH2 cytokine response followed by regulatory T-cell generation Abel Suárez-Fueyo, PhD, Tania.

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy Erik Wambre, PhD, Jonathan.

Josée Lamoureux, PhD, Jana Stankova, PhD, Marek Rola-Pleszczynski, MD

What is an “eosinophilic phenotype” of asthma?

Enhanced CD46-induced regulatory T cells suppress allergic inflammation after Dermatophagoides pteronyssinus–specific immunotherapy Yi-Giien Tsai, MD,

Autophagy: Nobel Prize 2016 and allergy and asthma research

Persistence of natural killer cells with expansion of a hypofunctional CD56−CD16+KIR+NKG2C+ subset in a patient with atypical Janus kinase 3–deficient.

Matthew J. Loza, PhD, Susan Foster, PhD, Stephen P

Sara Paveglio, PhD, MS, Erin Bennett, MS, Kelly L. Hawley, PhD, Adam P

Influence of cytomegalovirus infection on immune cell phenotypes in patients with common variable immunodeficiency Sayed Mahdi Marashi, PhD, Mohammed.

Allergen-specific immunotherapy modulates the balance of circulating Tfh and Tfr cells Véronique Schulten, PhD, Victoria Tripple, BSc, Grégory Seumois,

Inhibition of human allergic T-cell responses by IL-10–treated dendritic cells: Differences from hydrocortisone-treated dendritic cells Iris Bellinghausen,

IL-10–producing monocytes differentiate to alternatively activated macrophages and are increased in atopic patients Antje Prasse, MD, Martin Germann,

Ovalbumin-specific IgE modulates ovalbumin-specific T-cell response after repetitive oral antigen administration Nemuko Omata, MD, Yusei Ohshima, MD,

The novel synthetic immune response modifier R-848 (Resiquimod) shifts human allergen-specific CD4+ TH2 lymphocytes into IFN-γ–producing cells Francesca.

Common variable immunodeficiency is associated with a functional deficiency of invariant natural killer T cells Yifang Gao, PhD, Sarita Workman, MSc,

Alexander S. Kim, MD, Taylor A. Doherty, MD, Maya R

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites

Presentation transcript:

Perturbations of natural killer cell regulatory functions in respiratory allergic diseases Francesca Scordamaglia, MD, Mirna Balsamo, PhD, Antonio Scordamaglia, MD, Alessandro Moretta, MD, Maria Cristina Mingari, PhD, Giorgio Walter Canonica, MD, Lorenzo Moretta, MD, Massimo Vitale, PhD Journal of Allergy and Clinical Immunology Volume 121, Issue 2, Pages 479-485 (February 2008) DOI: 10.1016/j.jaci.2007.09.047 Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Circulating CD56++CD16+/− NK cells are reduced in allergic patients. Percentages of CD56+CD3− NK cells in PBL (A) and of CD56++ cells within the NK cell population (B) are shown (healthy individuals, squares; patients, triangles). C, CD56 and CD16 expression in purified NK cells from a healthy representative individual (H7) and a representative individual with allergy (P10). The CD56++CD16+/− cell population is quantified. Journal of Allergy and Clinical Immunology 2008 121, 479-485DOI: (10.1016/j.jaci.2007.09.047) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Evaluation of NK-cell–mediated IFN-γ production in patients with allergy or healthy subjects. A, IFN-γ content in supernatants from allergic (black) or healthy (gray) NK cells cultured with DCs. B and C, NK cells stimulated by cytokines are analyzed by FACS for their IFN-γ content (patients, black; healthy, gray). Journal of Allergy and Clinical Immunology 2008 121, 479-485DOI: (10.1016/j.jaci.2007.09.047) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 NK cells of patients with allergy induce poor DC maturation. Cytofluorimetric analysis of CD86 expression on DCs that have been cultured either in the absence (DC) or in the presence of healthy (DC+hNK) or patient (DC+pNK) NK cells. On the left and the right sides are indicated the DC and the P/H pairs analyzed in each experiment. Numbers indicate the CD86 mean fluorescence intensity. Journal of Allergy and Clinical Immunology 2008 121, 479-485DOI: (10.1016/j.jaci.2007.09.047) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Analysis of the ability of patients' NK cells to kill iDCs. Patients (black) and healthy (gray) NK cells are compared in a cytolytic assay for their ability to kill allogeneic iDCs. NK cells from each P/H pair were assessed against a different iDC target. E/T ratio, 10/1. Journal of Allergy and Clinical Immunology 2008 121, 479-485DOI: (10.1016/j.jaci.2007.09.047) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Synoptic data of the NK cell phenotype and functions of allergic patients analyzed. Diamonds, Percent of CD56++ NK cells. Bars, Patient/healthy donor (P/H) values ratio related to all of the NK cell functions analyzed (data from Fig 2, B, are not reported because they are not statistically significant). Black, IFN-γ induced on NK-DC coculture. Striped, DC killing. Gray, CD86 mean fluorescence intensity (DC maturation). White, IL-12 produced by DCs on NK-cell stimulation. The solid line refers to data in the healthy controls (P/H ratio = 1). The dotted line indicates the mean percentage of CD56++ cells in healthy controls. Journal of Allergy and Clinical Immunology 2008 121, 479-485DOI: (10.1016/j.jaci.2007.09.047) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions