Mutational signature analysis of WES data from patients with advanced PDAC. A, Projection of signatures representing four main mutational processes identified.

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Mutational signature analysis of WES data from patients with advanced PDAC. A, Projection of signatures representing four main mutational processes identified in de novo signature analysis. Mutational signature analysis of WES data from patients with advanced PDAC. A, Projection of signatures representing four main mutational processes identified in de novo signature analysis. All 71 samples in the cohort are listed as columns. Each row represents a signature as defined in the text: COSMIC1 (C>T transitions at CpG dinucleotides, Aging), COSMIC2 and 13 (APOBEC), COSMIC3 (HRD or BRCA deficient), and COSMIC17 (unknown). B, Samples are shown by the number of HRD/COSMIC3 mutations (y-axis) and binned according to whether they have a mutation or gene expression alteration in the known HR genes BRCA1, BRCA2, PALB2, or RAD51C (HRD altered; x-axis). Legend below indicates coloring based on type of alteration. RAD51C downregulation refers to more than 2-fold downregulation of mRNA expression levels below the mean value for the entire cohort. “No events” refers to no detected mutation, copy-number alteration, or mRNA downregulation in the genes indicated. C, Scatter plot of samples displayed by the number of large (≥9 base pairs) deletions on the y-axis and the number of HRD/COSMIC3 mutations on the x-axis. Coloring as shown in the legend below. Andrew J. Aguirre et al. Cancer Discov 2018;8:1096-1111 ©2018 by American Association for Cancer Research