Fig. 2 In situ extraction of miRNAs using the nanowire-anchored microfluidic device. In situ extraction of miRNAs using the nanowire-anchored microfluidic.

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Fig. 2 In situ extraction of miRNAs using the nanowire-anchored microfluidic device. In situ extraction of miRNAs using the nanowire-anchored microfluidic device. (A) Scatterplot of normalized intensities of miRNAs extracted from the collected EVs on nanowires in the device versus the ultracentrifuged EVs. Each point corresponds to a different miRNA type (that is, species). The boundary between pink and cyan represents the same level of miRNA expression for the two approaches. (B) Histogram of miRNA species for nanowires (pink) and ultracentrifugation extraction (cyan). Error bars show the SD for a series of measurements (n = 3). (C) Scatterplot of normalized intensities of miRNAs extracted from the collected EVs using the nanowire-anchored microfluidic device versus miRNA expression extracted from the collected EVs when using a commercially available kit. Each point corresponds to a different miRNA type (species). The boundary between pink and gray represents the same level of miRNA expression for the two approaches. (D) Histogram of miRNA species for nanowires (pink) and the commercially available kit (gray). Error bars show the SD for a series of measurements (n = 3). a.u., arbitrary units. Takao Yasui et al. Sci Adv 2017;3:e1701133 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).