Antigen-specific cytokine production detected by multiplex bead assay (Luminex) 3 weeks after the 3rd immunization. Antigen-specific cytokine production detected by multiplex bead assay (Luminex) 3 weeks after the 3rd immunization. Each group consisted of 4 mice, and error bars represent the standard deviations of the means. *, P < 0.05 versus CelTOS alone; #, P < 0.05 versus same % oil, with or without GLA; †, P < 0.05 versus GLA-AF; ‡, P < 0.05 versus Montanide. Christopher B. Fox et al. Clin. Vaccine Immunol. 2012; doi:10.1128/CVI.00235-12