Antigen-specific cytokine-producing cells (IFN-γ and IL-5) detected by ELISPOT assay 3 weeks after the 3rd immunization. Antigen-specific cytokine-producing.

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Antigen-specific cytokine-producing cells (IFN-γ and IL-5) detected by ELISPOT assay 3 weeks after the 3rd immunization. Antigen-specific cytokine-producing cells (IFN-γ and IL-5) detected by ELISPOT assay 3 weeks after the 3rd immunization. Each group consisted of 5 mice, and error bars represent the standard deviations of the means. Additional statistically significant differences (P < 0.05) that were not plotted include the following: for IFN-γ, 1% or 2% GLA-SE group versus SE group at same emulsion concentration, 1% or 2% GLA-SE versus Montanide, and 1% GLA-SE versus GLA-AF; and for IL-5, 2% SE versus 2% GLA-SE, GLA-AF, or Montanide. *, P < 0.05 versus CelTOS alone; §, P < 0.05 versus 2% SE. Christopher B. Fox et al. Clin. Vaccine Immunol. 2012; doi:10.1128/CVI.00235-12