Fig. 2 No evidence of HIV molecular evolution in diverse tissues obtained at autopsy (HAMB-1 and HAMB-2). No evidence of HIV molecular evolution in diverse.

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Fig. 2 No evidence of HIV molecular evolution in diverse tissues obtained at autopsy (HAMB-1 and HAMB-2). No evidence of HIV molecular evolution in diverse tissues obtained at autopsy (HAMB-1 and HAMB-2). PBMCs were obtained from individuals after years of cART, and tissue samples were taken at autopsy after these individuals expired. HIV pro-pol sequences were obtained and then aligned; neighbor-joining phylogenetic trees were constructed). Hypermutated sequences were removed, and root-to-tip distances were determined as in Fig. 1. HAMB-1 initiated cART early after infection, and HAMB-2 initiated ART during chronic infection (see the Supplementary Materials). HIV DNA sequences obtained at the time of autopsy did not diverge from HIV DNA sequences recovered from PBMCs years previously. HIV DNA sequences that were identical to HIV DNA sequences from the PBMCs were found in all the tissues sampled, including the lung, spleen, multiple lymph nodes, testes, and the frontal and occipital lobes of the brain taken at autopsy; as a result, there were numerous groups with identical sequences present from multiple anatomic locations and PBMCs (black arrows). G. Bozzi et al. Sci Adv 2019;5:eaav2045 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).