Human BAT in allergic and normal control subjects.

Slides:

Advertisements

Similar presentations

A. a). d). c). f). b).e). g). Figure S1. NAT2 expression in lymphocytes and monocytes from a healthy volunteer. PBMC from a healthy volunteer were simple.

Advertisements

Flow Cytometry. Allows For Detection Of Surface Markers Of Cells Allows For Detection Of Intracellular Factors Allows Detection Of Secreted Factors By.

The expression and signaling of FGFR3 in WM cells.

Copyright © 2014 American Medical Association. All rights reserved.

Fig. 2. Comparison of the Th1/Th2 ratio and the percentages of Th1 and Th2 cells between normal and allergic individuals. (A) The Th1/Th2 ratio observed.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut Claudia.

Image 1 Detection of minimal residual disease (MRD) in consecutive bone marrow (BM) samples from a patient with relapse (A) and a patient still in remission.

Figure 2 Percentage of CD19+/CD34+ cells with decreased CD81 median fluorescence intensity (MFI;

A B C Day 0 Side scattering Forward scattering Day 0 Day 10

Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu Amanda J. Reefer, MS, Kathryn.

Margaretha A. Faber, MD, Vito Sabato, MD, Chris H

Role of γ/δ T cells in a patient with CD4+ CD3– lymphocytosis, hypereosinophilia, and high levels of IgE Ilan Bank, MDa,d, Avner Reshef, MDb, Miriam.

Reduced endothelial progenitor cells in extracranial arterial stenosis but not intracranial arterial stenosis Zhixin Huang, MD, PhD, Xiaohao Zhang, MD,

A B C Supplementary Figure 1 Group 1 Group 2 Group 3 #1 #2 #3 #4 #5 #6

Delayed clinical and ex vivo response to mammalian meat in patients with IgE to galactose-alpha-1,3-galactose Scott P. Commins, MD, PhD, Hayley R. James,

Further Analysis of Interleukin-2 Receptor Subunit Expression on the Different Human Peripheral Blood Mononuclear Cell Subsets by Denis David, Lynda Bani,

Antigen-driven basophil activation is indicative of early Necator americanus infection in IgE-seronegative patients Franco H. Falcone, PhD, Gary Telford,

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut Claudia.

Recruitment of myeloid and plasmacytoid dendritic cells in cervical mucosa during Chlamydia trachomatis infection T. Agrawal, V. Vats, P.K. Wallace,

The inhibitory effects of topically active glucocorticoids on IL-4, IL-5, and interferon-γ production by cultured primary CD4+ T cells Shelby P. Umland,

BÜHLMANN CAST® Assays Technical Presentation

Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis Kaori Mukai, PhD, Nicolas Gaudenzio,

Residual Cancer Lymphocytes in Patients With Chronic Lymphocytic Leukemia After Therapy Show Increased Expression of Surface Antigen CD52 Detected Using.

CD34+ Cells in the Peripheral Blood Transport Herpes Simplex Virus DNA Fragments to the Skin of Patients with Erythema Multiforme (HAEM) Fumitake Ono,

Omalizumab treatment downregulates dendritic cell FcεRI expression

Volume 16, Issue 2, Pages (February 2002)

Intraoperative detection of lymph node micrometastasis with flow cytometry in non– small cell lung cancer Manabu Ito, MD, Yoshihiro Minamiya, MD, PhD,

Antibody conjugates bispecific for intercellular adhesion molecule 1 and allergen prevent migration of allergens through respiratory epithelial cell layers

Mass cytometry profiling the response of basophils and the complete peripheral blood compartment to peanut Leticia Tordesillas, PhD, Adeeb H. Rahman,

Increased Soluble CD226 in Sera of Patients with Cutaneous T-Cell Lymphoma Mediates Cytotoxic Activity against Tumor Cells via CD155 Naomi Takahashi,

In vivo allergenic activity of a hypoallergenic mutant of the major fish allergen Cyp c 1 evaluated by means of skin testing Nikolaos Douladiris, MD,

TACI mutation with invasive polyclonal CD8+ T-cell lymphoproliferation in a patient with common variable immunodeficiency Lucinda J. Berglund, MBBS,

Mark Gorelik, MD, Satya D. Narisety, MD, Anthony L

Volume 132, Issue 1, Pages (January 2007)

Plasma levels of microparticles at 24 weeks of gestation do not predict subsequent pregnancy complications Ophira Salomon, M.D., Ben-Zion Katz, Ph.D.,

β-Agonist enhances type 2 T-cell survival and accumulation

Schematic representation of Anisakis simplex pathogenicity in the alimentary tract. Schematic representation of Anisakis simplex pathogenicity in the alimentary.

Identification and characterization of stemlike cells in human esophageal adenocarcinoma and normal epithelial cell lines Ronghua Zhao, MD, PhD, Luca.

The Relationship of MHC-Peptide Binding and T Cell Activation Probed Using Chemically Defined MHC Class II Oligomers Jennifer R Cochran, Thomas O Cameron,

Abnormal Natural Killer Cell Function in Systemic Sclerosis: Altered Cytokine Production and Defective Killing Activity Mayuka Horikawa, Minoru Hasegawa,

AID Is Required for c-myc/IgH Chromosome Translocations In Vivo

Individual IL-3 priming is crucial for consistent in vitro activation of donor basophils in patients with chronic urticaria Thomas Gentinetta, MSc, Tatjana.

Volume 117, Issue 3, Pages (September 1999)

Peanut T-cell epitope discovery: Ara h 1

Heterogeneity of mast cells and T cells in the nasal mucosa

(A) HLA-E expression on the surface of CD14+ cells in patients with AS, HLA-B*2705, HLA-B*2709-positive and HLA-B27-negative HCs. Results are expressed.

Volume 68, Issue 2, Pages (August 2005)

Oktay Kirak, MD, Gert Riethmüller, MD

Attributions of anaphylaxis before and after screening for anti-Anisakis simplex responses. Attributions of anaphylaxis before and after screening for.

Residual Cancer Lymphocytes in Patients With Chronic Lymphocytic Leukemia After Therapy Show Increased Expression of Surface Antigen CD52 Detected Using.

Epigallocatechin gallate, the main component of tea polyphenol, binds to CD4 and interferes with gp120 binding Kazushige Kawai, MD, Nelson H Tsuno, MD,

Defining antigen-specific responses with human MHC class II tetramers

Toll-like receptor 2 ligands activate human basophils for both IgE-dependent and IgE- independent secretion Anja P. Bieneman, BS, Kristin L. Chichester,

Comparison of conventional NSG and hIL-7 expressing NSG humanized mice

Increasing prevalence of multidrug resistance among P

Volume 8, Issue 2, Pages (July 2014)

Clinical safety of Food Allergy Herbal Formula-2 (FAHF-2) and inhibitory effect on basophils from patients with food allergy: Extended phase I study

Systemic and localized seminal plasma hypersensitivity patients exhibit divergent immunologic characteristics Debajyoti Ghosh, PhD, Jonathan A. Bernstein,

Eric B. Brandt, PhD, Melissa K. Mingler, MS, Michelle D

Fabrizio Dutly, and Martin Altwegg Clin. Microbiol. Rev. 2001; doi:10

CD11b-mediated migratory property of peripheral blood B cells

Intracellular TNF-α and IL-4 expression in gated CD33+ monocytes by three-color flow cytometry. Intracellular TNF-α and IL-4 expression in gated CD33+

Flow cytometric analysis of T cell activation.

Diagnostic testing and interpretation of tests for autoimmunity

Melanoma patient monocytes have altered expression of inflammatory and surface markers. Melanoma patient monocytes have altered expression of inflammatory.

Flow cytometry analysis of intracellular cytokine expression in control PBMC (left set of panels) and following either PMA-ionomycin activation (middle.

Fig. 4 S. elongatus therapy does not elicit a pathologic immune response nor persist in tissue long-term. S. elongatus therapy does not elicit a pathologic.

Correlation of HIV-1 replication fitness, as measured by the Monogram Biosciences RC assay, with baseline CD4+ T-cell count (a) and baseline plasma HIV-1.

The Relationship of MHC-Peptide Binding and T Cell Activation Probed Using Chemically Defined MHC Class II Oligomers Jennifer R Cochran, Thomas O Cameron,

Surface CD4 and CD14 and intracellular TNF-α expression in PBMCs

Presentation transcript:

Human BAT in allergic and normal control subjects. Human BAT in allergic and normal control subjects. Activation of basophils was detected by flow cytometry using a fluorophore-tagged (phycoerythrin [PE]) MAb against the CD63 cell activation marker. The expression of CD63 (horizontal axis) is plotted against levels of anti-IgE fluorescein isothiocyanate (FITC) (vertical axis) in response to A. simplex crude extract. The left-hand plot shows results from the first patient described 11 years before (25), although the test was carried out 11 years later (2006), and the right-hand panel corresponds to a female subject exhibiting no allergy to A. simplex used as a control. The results are expressed as the percentage of CD63+ basophils. Note that activated basophils from the allergic patient appear in the upper right window encompassing 98% of marked cells. In contrast, the control (right-hand plot) shows that a mere 3% of basophils were activated upon exposure to parasite antigen/allergens. (M. T. Audicana and N. Longo, unpublished data.)‏ M. Teresa Audicana, and Malcolm W. Kennedy Clin. Microbiol. Rev. 2008; doi:10.1128/CMR.00012-07