Brushed Aside and Silenced

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Brushed Aside and Silenced Lyubomira Chakalova, Peter Fraser Developmental Cell Volume 14, Issue 4, Pages 461-462 (April 2008) DOI: 10.1016/j.devcel.2008.03.020 Copyright © 2008 Elsevier Inc. Terms and Conditions

Figure 1 Genes Tethered to the Inner Nuclear Membrane Are Transcriptionally Suppressed A gene construct tagged with LacO sites is stably integrated and expressed in mammalian cells. A GFP-LacI-EMD fusion protein is stably coexpressed in the cells. The protein is tethered to the inner nuclear membrane by the EMD segment. In the presence of IPTG, LacI is unable to bind to its target LacO sites and the reporter gene is located in the nucleoplasm (before targeting). The gene can be targeted to the nuclear periphery by removal of IPTG (after targeting). The expression of the gene is suppressed when the gene is tethered to the periphery. Developmental Cell 2008 14, 461-462DOI: (10.1016/j.devcel.2008.03.020) Copyright © 2008 Elsevier Inc. Terms and Conditions