Fig. 4 No genetic divergence of HIV during cART.

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Fig. 4 No genetic divergence of HIV during cART. No genetic divergence of HIV during cART. HIV sequences were subjected to root-to-tip analysis to investigate genetic divergence during cART. To calculate root-to-tip distances, a consensus HIV sequence was constructed for each individual from the earliest available time point, and root-to-tip distances were determined for HIV sequences from each time point as described in Materials and Methods. No divergence was detected during cART for AVBIO2-15, AVBIO2-04, HAMB-1, and HAMB-2. For AVBIO2-35 and AVBIO2-37, genetic divergence was detected during suboptimal therapy; upon initiating cART with suppression of viremia, no further divergence was detected. A decrease in root-to-tip distances was detected comparing samples obtained in plasma prior to cART and HIV DNA in samples obtained during suppressive cART because of the persistence of HIV DNA sequences from the pre-cART period. G. Bozzi et al. Sci Adv 2019;5:eaav2045 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).