ERM proteins promote CD44 cleavage.

Slides:

Advertisements

Similar presentations

Constitutive NF-κB activation by the t(11;18)(q21;q21) product in MALT lymphoma is linked to deregulated ubiquitin ligase activity Honglin Zhou, Ming-Qing.

Advertisements

miR-133a positively regulated p53/p21 pathway.

Pirh2 promotes p73 ubiquitination in vivo.

Proline supplementation during P5CS protein knockdown suppressed GCN2 activation. Proline supplementation during P5CS protein knockdown suppressed GCN2.

NF1 downregulation activates MAPK pathway signaling.

Fig. 2 Fas controls IL-1RA–sEV secretion in murine MSCs.

Increased Ezrin Expression and Activation by CDK5 Coincident with Acquisition of the Senescent Phenotype Hai-Su Yang, Philip W Hinds Molecular Cell

Downregulation of SPRY4 expression is associated with FGFR1–FRS2 activation. Downregulation of SPRY4 expression is associated with FGFR1–FRS2 activation.

Interfering with HMGA2 expression blocks transformation in metastatic lung cancer cells. Interfering with HMGA2 expression blocks transformation in metastatic.

Expression of SYCE2 inhibits the interaction of HP1α with H3K9me3.

XIST regulated miR-29c by directly targetting in TMZ-resistant glioma cells XIST regulated miR-29c by directly targetting in TMZ-resistant glioma cells.

Collagen-dependent phosphorylation of eIF4E in PDAC cells.

HOXB7 interacts with ERα and enhances expression of ER target genes.

BRAF inhibitor-resistant BRAF-mutant melanoma cells show increased invasion and metastatic potential. BRAF inhibitor-resistant BRAF-mutant melanoma cells.

Tyrosine phosphorylations of both DAB1 and ABL are essential for invasion of colorectal cancer cells. Tyrosine phosphorylations of both DAB1 and ABL are.

The role of SRC-C3G-RAP1 signaling in transformation induced by CRKL

ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. (A) Western.

Specific inhibition of Ras, but not Rac, inhibits EGF-stimulated protrusion. Specific inhibition of Ras, but not Rac, inhibits EGF-stimulated protrusion.

JAK3A572V mutation causes constitutive JAK3 activity and IL-2–independent proliferation of NKTCL cells. JAK3A572V mutation causes constitutive JAK3 activity.

The p53 pathway is involved in the inhibition of cell proliferation observed in 15-LOX-1-overexpressing cells. The p53 pathway is involved in the inhibition.

CREB1 promotes the induction of endogenous ERα target genes.

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

MiR-431-5p directly targets RAF1 and is negatively correlated to RAF1 expression (A) The predicted binding sites of miR-431-5p and RAF1 3′-UTR, and mutant.

Decreased KATP activity for SUR1 (ABCC8) channels harboring R1420H or R1420C mutations. Decreased KATP activity for SUR1 (ABCC8) channels harboring R1420H.

Fig. 1 BMS binds to the pseudokinase domain of TYK2 and inhibits receptor-mediated TYK2 activation and downstream phosphorylation events in human.

Effect of GSK-3β inhibition on cell-confluence-induced apoptosis.

PKM2 is tyrosine phosphorylated and inhibited by FGFR1 in cancer cells with oncogenic or overexpressed FGFR1. PKM2 is tyrosine phosphorylated and inhibited.

Targeting eIF4E increases ZEB1 protein and mRNA levels and decreases ZEB1-targeting miR-200c and miR-141 microRNAs. Targeting eIF4E increases ZEB1 protein.

Induction of CDCP1 is regulated by Ras in NSCLC cells.

CDDO-Me induces apoptosis independent of Bcl-2 level as well as p53 status in human NSCLC cells. CDDO-Me induces apoptosis independent of Bcl-2 level as.

SATB2-AS1 repressed Snail transcription depending on SATB2-mediated recruitment of HDAC1. SATB2-AS1 repressed Snail transcription depending on SATB2-mediated.

SiRNA silencing of CDH3, CLDN1, KRT23, and MMP7 (A–D) in adenoma and CRC cell lines significantly reduces cell proliferation. siRNA silencing of CDH3,

RhoH reconstitution in HCL represses CD11c promoter activity.

Effect of expressing an activated mutant (Rac V12) and an inactivated mutant (Rac N17) of Rac on PTHRP gene expression levels and on JNK activation. Effect.

Expression of CRC stem cell markers and L1 in CRC cells.

Knockdown of PKM2 suppressed TPA-induced skin cell transformation.

Effect of SFN on the protein expression of Nrf2, HO-1, and NQO1 in JB6-shMock and JB6-shNrf2 cells. Effect of SFN on the protein expression of Nrf2, HO-1,

TFAP2A knockdown inhibits cell growth by targeting HIF-1α signaling.

Overexpression of EP4 with cAMP-PKA signal activation promoted the castration-resistant progression of LNCaP cells through AR activation. Overexpression.

Targeting DCLK1 by miRNA-137.

Activation of Wnt signaling pathway in trastuzumab-resistant cell lines. Activation of Wnt signaling pathway in trastuzumab-resistant cell lines. A, bar.

Effect of GSK3β inhibition on cell survival and proliferation of glioblastoma cells. Effect of GSK3β inhibition on cell survival and proliferation of glioblastoma.

CDCP1 links Ras and SFK signaling and regulates anoikis resistance, cell migration, and invasion in NSCLC cells. CDCP1 links Ras and SFK signaling and.

Neurotensin and insulin induce increases in CTGF, CYR61, and CXCL5 gene expression and stimulate cell proliferation and colony via YAP/TAZ in PDAC cells.

Ki-67 expression in M31- and H3-treated tumors (A) and respective Ki-67 labeling indices in the two groups of tumors (B). Ki-67 expression in M31- and.

Expression of dominant-negative RasN17 completely suppresses Ras activation in Rh1 cells. Expression of dominant-negative RasN17 completely suppresses.

Potential model of HMQ1611 inhibiting breast cancer cell proliferation

Bim expression and activation correlates with reduced Notch3 expression and increased anoikis. Bim expression and activation correlates with reduced Notch3.

Changes in signal transduction pathway induced by gefitinib.

HES1-dependent activation of SRC/STAT3 pathway is mediated by transcription-independent mechanism. HES1-dependent activation of SRC/STAT3 pathway is mediated.

Substitution of endogenous Reptin with D299N mutant leads to HuH7 cell growth arrest. Substitution of endogenous Reptin with D299N mutant leads to HuH7.

Tumor-resident CD8+ T cells rapidly expand after IL-15/IL-15Rα complex treatment. Tumor-resident CD8+ T cells rapidly expand after IL-15/IL-15Rα complex.

Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Expression.

Transcriptional activity and growth of mutant ER in a breast cancer cell line. Transcriptional activity and growth of mutant ER in a breast cancer cell.

The critical roles of miR-23a and miR-27a in colorectal cancer progression. miR-23a primarily increases cell motility through downregulation of its target.

Comparison of Akt1 and Akt3 for their abilities to activate the Fra-1 promoter. Comparison of Akt1 and Akt3 for their abilities to activate the Fra-1 promoter.

HOXA11-AS acts as a ceRNA for miR-1297.

Decreased dopamine uptake following downregulation of SLC6A3.

The interaction of PALB2 with BRCA1 is required for the assembly of PALB2, BRCA2, and RAD51 nuclear foci. The interaction of PALB2 with BRCA1 is required.

Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization in 231BR3 cells. Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization.

PARP1 suppresses the transcription of PD-L1 in cancer cells.

RRP1B interacts with TRIM28 and HP1α at regions associated with H3K9me3 and decreased gene expression. RRP1B interacts with TRIM28 and HP1α at regions.

Expression of chemokine receptors in A-498 cell line.

Wild-type and oncogenic Ras differentially regulate cell proliferation

Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.

Mechanism of ERM protein–dependent CD44 cleavage.

CREB1 binds at the proximal region of the TGFB2 promoter and induces its transcriptional activation. CREB1 binds at the proximal region of the TGFB2 promoter.

D-GM3 promotes uPAR clustering on the cell surface and activates p38 MAPK. A, uPAR expression in cells was either knocked down by treatment with 4 independent.

Sp1 complementation assay.

An NH2-terminal domain of PALB2, distinct from the BRCA2-interacting domain of PALB2, mediates interaction with BRCA1 and assembly of PALB2 nuclear foci.

Presentation transcript:

ERM proteins promote CD44 cleavage. ERM proteins promote CD44 cleavage. A, downregulation of all 3 ERM proteins reduce CD44 cleavage. RPM-MC cells were transfected as in Fig. 1A. Cells were grown at low density. The expression of ERM proteins was downregulated in about 98% of the cells by a mixture of siRNAs targeting all 3 members of the ERM protein family, ezrin, radixin, and moesin (nontargeting siRNA “C” was used as control). Downregulation of ERM proteins inhibited ectodomain cleavage of CD44. B, NRG1 cleavage is resistant to downregulation of ERM proteins. Setup of the experiment as in A, except that double-tagged NRG1 was transfected as cleavage substrate. C, constitutive activation of the Ras pathway does not stimulate CD44 cleavage. Overexpression of an HA-tagged dominant active SOS mutant (DA-SOS, 24) that is permanently membrane-associated, activates Ras—as detected by phosphorylated Erk—independently of the presence of ERM proteins and TPA treatment. Histogram shows mean values of relative level of solCD44E ± SD from 3 independent experiments (Wt: V vs. DA-SOS, P = 0.53955; KR-Mt: V vs. DA-SOS, P = 0.932614). Monika Hartmann et al. Mol Cancer Res 2015;13:879-890 ©2015 by American Association for Cancer Research