Quercetin induces autophagy, blocks cell cycle and activation of ERK and JNK signaling pathways participates in the G1 arrest in P39 cells. Quercetin induces.

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Cell Physiol Biochem 2014;33: DOI: /

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Quercetin induces autophagy, blocks cell cycle and activation of ERK and JNK signaling pathways participates in the G1 arrest in P39 cells. Quercetin induces autophagy, blocks cell cycle and activation of ERK and JNK signaling pathways participates in the G1 arrest in P39 cells. After treatment cell-cycle distribution (A) according to their DNA content cyclins (B), Cdks (C) related to G1 phase. D, Cdks inhibitors CKI (p21 and p27) and p-Rb (E) ERK (thr183/tyr187) and JNK (thr183/tyr185). P39 cells were pretreated for 1 hour with SP600125 (20 μmol/L) and/or PD184352 (20 μmol/L) and treated with 50 μmol/L of quercetin for 24 hours. After treatment, cells were stained with Annexin V/PI (F) or with PI (G), the percentage of apoptotic cells and the cell-cycle distribution according to their DNA content in G1 phase and acidic vesicular organelles stained with AO (H), respectively, were determined by flow-cytometry analysis. I, PI3K class III, Beclin-1, Atg5-Atg12, Atg7, LC3 I/II cleaved expression (J) phosphorylation of Akt (Ser 473) and mTOR (Ser 2448) were determined by Western blotting analysis. P39 cells were pretreated for 1 hour with chloroquine (20 μmol/L) and treated with 50 μmol/L of quercetin for 24 hours. After treatment cells were stained with Annexin V/PI (K) or with PI (L), the percentage of apoptotic cells and cell-cycle distribution according to their DNA content in G1 phase, respectively, were determined by flow-cytometry analysis. All data were representative of three independent experiments.*, P < 0.05; **, P < 0.0001; #, P < 0.05 versus quercetin. Victor Maso et al. Cancer Prev Res 2014;7:1240-1250 ©2014 by American Association for Cancer Research