Fig. 2 Purification and characterization of the five RumC isoforms produced in vivo. Purification and characterization of the five RumC isoforms produced.

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Fig. 2 Purification and characterization of the five RumC isoforms produced in vivo. Purification and characterization of the five RumC isoforms produced in vivo. (A) Protocol for extraction from cecal contents to obtain a purified mixture of RumCs. Fractions were selected on the basis of their anti-Cp activity throughout the purification steps. PBS, phosphate-buffered saline. (B and C) LC-MS analyses of the two fractions containing the different RumC (* and † indicate succinimide and deamidated forms of C5 and C3, respectively). RumC5 was present in two consecutive C18 reversed-phase fractions. (D) Deconvoluted mass spectrum of RumC1 eluted at 21.6 min in nano–LC-MS analyses. (E) Deconvoluted mass spectrum of RumC1 after dithiothreitol (DTT)/iodoacetamide treatment. (F) Deconvoluted mass spectrum of synthetic unmodified RumC1. Steve Chiumento et al. Sci Adv 2019;5:eaaw9969 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).