Analysis of dCas9 expression.

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Analysis of dCas9 expression. Analysis of dCas9 expression. (A) Growth analysis of wild-type L. plantarum WCFS1 (blue), IM133 (WCFS1 carrying pSIP-SH-dCas9 and pSgRNA-notarget; gray), IM132 (WCFS1 carrying only pSIP-SH-dCas9; orange), and IM167 (WCFS1 carrying pEV and pSgRNA-notarget; green). Levels of growth of noninduced cultures and induced cultures (25 ng/ml inducer pheromone SppIP; black outline) are shown. (B) Phase-contrast micrographs of wild-type L. plantarum WCFS1 cells and IM133. The scale bars represent 5 µm. Ine Storaker Myrbråten et al. mSphere 2019; doi:10.1128/mSphere.00007-19