Memory phenotypes PBMC-derived MERS-CoV-specific T cells from CWs

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Memory phenotypes PBMC-derived MERS-CoV-specific T cells from CWs Memory phenotypes PBMC-derived MERS-CoV-specific T cells from CWs. PBMCs from seropositive (CW16) and seronegative (CW14) subjects were stimulated with MERS-CoV structural protein-specific peptide pools for 12 h in the presence of brefeldin A. IFN-γ+ TNF+ virus-specific CD4 (A, B) and CD8 (C, D) T cells were analyzed for CD45RA expression. Memory phenotypes PBMC-derived MERS-CoV-specific T cells from CWs. PBMCs from seropositive (CW16) and seronegative (CW14) subjects were stimulated with MERS-CoV structural protein-specific peptide pools for 12 h in the presence of brefeldin A. IFN-γ+ TNF+ virus-specific CD4 (A, B) and CD8 (C, D) T cells were analyzed for CD45RA expression. Abeer N. Alshukairi et al. mBio 2018; doi:10.1128/mBio.01985-18