Fig. 2. Effects of CB_36 and its analogs in HepG2 cells

Slides:

Advertisements

Similar presentations

BEL7402Huh7 C Tet Rapa C Tet Rapa LC3-II LC3-I GAPDH Figure S1 Detecting Tetrandrine-induced autophagy in Huh7 and BEL7402 cell lines. Rapamycin treatment.

Advertisements

Date of download: 6/23/2016 Copyright © The American College of Cardiology. All rights reserved. From: Discovery of a New Role of Human Resistin in Hepatocyte.

Glioma-derived soluble factor(s) enhance IL-10 signaling.

Differential effects of GLP-1 receptor agonist on foam cell formation in monocytes between non-obese and obese subjects Masashi Tanaka, Yoshiyuki Matsuo,

Suppressive effects of polyozellin on TGFBIp-mediated septic responses in human endothelial cells and mice Byeongjin Jung, Eun-Ju Yang, Jong-Sup Bae

Oxidized low-density lipoprotein (ox-LDL) binding to lectin-like ox-LDL receptor-1 (LOX- 1) in cultured bovine articular chondrocytes increases production.

Calcium spirulan derived from Spirulina platensis inhibits herpes simplex virus 1 attachment to human keratinocytes and protects against herpes labialis

Fig. 2. The effect of miR-379 on cell proliferation, invasion, migration, and apoptosis of VSMCs. Twenty-four hours after cells were transfected with miR-379.

Effect of Low Cholesterol on Steroid Hormones and Vitamin E Levels

by Kathryn Lagrue, Alex Carisey, David J

Wei Zhou, MD, Hong Chai, MD, PhD, Andy Courson, BS, Peter H

by Martha B. Johnson, and Caroline A. Enns

Glutamate signaling in chondrocytes and the potential involvement of NMDA receptors in cell proliferation and inflammatory gene expression T. Piepoli,

Aurora Kinase A Is Upregulated in Cutaneous T-Cell Lymphoma and Represents a Potential Therapeutic Target Daniel Humme, Ahmed Haider, Markus Möbs, Hiroshi.

Volume 128, Issue 1, Pages (January 2005)

Uptake of T-MPs for DC maturation and antigen presentation.

Volume 118, Issue 4, Pages (April 2000)

Jonathan C. Cohen, PhD Journal of Clinical Lipidology

Human Keratinocytes Express Functional CD14 and Toll-Like Receptor 4

An Essential Role of Hrs/Vps27 in Endosomal Cholesterol Trafficking

Bay induced aggregation of Cp occurs in HBV-infected HepG2-NTCP cells.

Volume 20, Issue 5, Pages (November 2014)

Molecular Therapy - Nucleic Acids

Fig. 3 BX795 blocks the synthesis of HSV-1 virions.

Simvastatin pretreatment enhances ischemia-induced neovascularization and blood flow recovery in streptozotocin-treated mice Po-Hsun Huang, MD, PhD,

Suppressive effect of CD39+CD73+ melanoma cells on T-cell proliferation, and reversion of this effect via treatment with the CD39-blocking antibody, CD39.

Shu-Jung Chang, Jeongmin Song, Jorge E. Galán Cell Host & Microbe

Oxidized low-density lipoprotein (ox-LDL) binding to lectin-like ox-LDL receptor-1 (LOX- 1) in cultured bovine articular chondrocytes increases production.

Midkine is expressed by infiltrating macrophages in in-stent restenosis in hypercholesterolemic rabbits Hiroshi Narita, MD, Sen Chen, PhD, Kimihiro Komori,

Terameprocol (Tetra-O-Methyl Nordihydroguaiaretic Acid), an Inhibitor of Sp1-Mediated Survivin Transcription, Induces Radiosensitization in Non-small.

Hypertonicity stimulates PGE2 signaling in the renal medulla by promoting EP3 and EP4 receptor expression Jeong-Ah Kim, Mee Rie Sheen, Sang Do Lee, Ju-Young.

The EGF receptor confers BRAF inhibitor resistance in BRAF-mutant melanoma cells. The EGF receptor confers BRAF inhibitor resistance in BRAF-mutant melanoma.

Figure 1 Statins, ezetimibe, and PCSK9 inhibitors all increase the expression of LDL receptors and reduce LDL-cholesterol levels (by percentages shown)

Response of monocytes to hY4 delivery and cytokine levels in CLL serum

Transcription Factor MIZ-1 Is Regulated via Microtubule Association

Fig. 1. Drug combination screen identifies BETi as acting synergistically with PARPi. Drug combination screen identifies BETi as acting synergistically.

Fig. 1 BX795 suppresses HSV-1 infection.

Glutamate signaling in chondrocytes and the potential involvement of NMDA receptors in cell proliferation and inflammatory gene expression T. Piepoli,

Volume 19, Issue 7, Pages (July 2012)

VAP silencing restores a normal plasma membrane cholesterol level in STARD3‐expressing cells VAP silencing restores a normal plasma membrane cholesterol.

Fig. 7 DMN-Tre labeling of Mycobacterium tuberculosis is inhibited by tuberculosis drug cocktail, unlike auramine staining. DMN-Tre labeling of Mycobacterium.

PC194 Ectopic Expression of Proprotein Convertase Subtilisin Kexin Type 9 by Smooth Muscular Cells Contributes to Aortic Dissection Yasunori IIda, Hiroki.

STARD3‐mediated cholesterol accumulation in endosomes occurs at the expense of plasma membrane STARD3‐mediated cholesterol accumulation in endosomes occurs.

Enhancement of receptor endocytosis, as well as cellular apoptosis, may explain the cooperative anti‐tumor actions of 3×mAbs and osimertinib Enhancement.

Inhibitor Mediated Protein Degradation

Volume 17, Issue 12, Pages (December 2010)

Volume 5, Issue 4, Pages (November 2013)

Fig. 5 DMN-Tre labeling is selective for live mycobacteria.

SiRNA knockdown of dynein IC2-C recovered the inhibition of neurite outgrowth in NF1-KD PC12 cells. siRNA knockdown of dynein IC2-C recovered the inhibition.

Calcium spirulan derived from Spirulina platensis inhibits herpes simplex virus 1 attachment to human keratinocytes and protects against herpes labialis

Insights in the Understanding of Cholesterol Metabolism:

Fibrinolytic activity of human mesothelial cells is counteracted by rapid uptake of tissue- type plasminogen activator Thomas Sitter, Karin Toet, Paul.

Infectious delivery of a 135-kb LDLR genomic locus leads to regulated complementation of low-density lipoprotein receptor deficiency in human cells Richard.

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 1. TSA at 165 nM induces maximal acetylation of histone H3 and near-maximal acetylation of histone H4.Immunoblot analysis of acetylated histones H3.

Fig. 1. Mitotic arrest results in differential RNA association with coilin.Untreated or nocodazole treated HeLa cell lysate was used for RNA immunoprecipitations.

Fig. 4 Labeling of Msmeg with DMN-Tre is fast and specific and depends on Ag85A function. Labeling of Msmeg with DMN-Tre is fast and specific and depends.

A Protein Transduction Domain with Cell Uptake and Selectivity Profiles that Are Controlled by Multivalency Effects Sandra M. DePorter, Irene Lui, Utpal.

Volume 93, Issue 2, Pages (April 1998)

MYC degradation screen identifies a compound that stabilizes MYC protein. MYC degradation screen identifies a compound that stabilizes MYC protein. (A)

Effective TcdB cell interactions correlate with endocytosis.

Fig. 2. Inhibition of insulin-like growth factor (IGF)-1-induced (pro)inflammatory cytokines of SZ95 sebocytes by eupatilin. With the exception of the.

Fertility and Sterility

Neuropeptide signaling through neurokinin-1 and neurokinin-2 receptors augments antigen presentation by human dendritic cells Junya Ohtake, PhD, Shun.

Effect of sulindac and atorvastatin on the cell cycle.

Fig. 3. In vivo effects of CB_36 in wild-type and Pcsk9 knockout mice

ALT-803 stimulates proliferation and activation of human NK cells and T cells in vitro. ALT-803 stimulates proliferation and activation of human NK cells.

Effect of nivolumab or ipilimumab on immune responses to vaccination in cynomolgus monkeys. Effect of nivolumab or ipilimumab on immune responses to vaccination.

Interleukin-17 is Produced by Both Th1 and Th2 Lymphocytes, and Modulates Interferon-γ- and Interleukin-4-Induced Activation of Human Keratinocytes Cristina.

SSTC-104 and LRP6 depletion effects on β-catenin and synovial sarcoma cells. SSTC-104 and LRP6 depletion effects on β-catenin and synovial sarcoma cells.

Presentation transcript:

Fig. 2. Effects of CB_36 and its analogs in HepG2 cells Fig. 2. Effects of CB_36 and its analogs in HepG2 cells. (A) At 18 h after treatment of CB_36, amounts of LDLR and PCSK9 were determined by immunoblot analysis. (B) Fluorescence-labeled Dil-LDL was incubated for an additional 2 h, and the uptake of Dil-LDL was analyzed by fluorescence microscopy. (C) The intensity of fluorescence was quantitated by flow cytometry analysis. Each value represents the ratio of the mean fluorescence intensity relative to that in vehicle-treated cells (DM). Error bars represent the SD of triplicate reactions. Similar results were obtained from at least three independent experiments. *p<0.05, †p<0.01 Student's t-test when compared with values in DMSO-treated cells. PCSK9, proprotein convertase subtilisin/kexin type 9; LDL, low density lipoprotein; LDLR, LDL receptor; GAPDH, glyceraledhyde-3-phosphate dehydrogenase; DMSO, dimethyl sulfoxide. Fig. 2. Effects of CB_36 and its analogs in HepG2 cells. (A) At 18 h after treatment of CB_36, amounts of LDLR and PCSK9 were determined by immunoblot analysis. (B) Fluorescence-labeled Dil-LDL was incubated for an additional 2 h, and the uptake of Dil-LDL was analyzed by fluorescence microscopy. (C) The intensity of fluorescence was quantitated by flow cytometry analysis . . . Yonsei Med J. 2015 Sep;56(5):1251-1257. http://dx.doi.org/10.3349/ymj.2015.56.5.1251