Negative effect of the use of the SuperScript III Platinum One-Step quantitative RT-PCR kit on the specificity of the MeVA RT-qPCR for the vaccine genotype.

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Negative effect of the use of the SuperScript III Platinum One-Step quantitative RT-PCR kit on the specificity of the MeVA RT-qPCR for the vaccine genotype. Negative effect of the use of the SuperScript III Platinum One-Step quantitative RT-PCR kit on the specificity of the MeVA RT-qPCR for the vaccine genotype. (A) The use of SuperScript III on the Roche LightCycler 480 platform caused a significant rise in the baseline of the amplification curves for genotype D10, D8, and B3. (B) Results of the use of SuperScript III on an ABI 7500 platform in amplification curves from wild-type measles virus RNA. Felicia Roy et al. J. Clin. Microbiol. 2017; doi:10.1128/JCM.01879-16