Fig. 5 Product analog 1,6-hexanediol at the substrate access cavity near the di-iron center and the cavity opening in the MMOH-MMOD complex. Product analog.

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Presentation transcript:

Fig. 5 Product analog 1,6-hexanediol at the substrate access cavity near the di-iron center and the cavity opening in the MMOH-MMOD complex. Product analog 1,6-hexanediol at the substrate access cavity near the di-iron center and the cavity opening in the MMOH-MMOD complex. (A) Composite omit map [1.5σ contour, calculated using the PHENIX software package (38)] of MMOH-MMOD near the di-iron center. The electron density map is shown as a mesh (gray), and 1,6-hexanediol and formate are colored green. (B) Substrate access cavity in the M. trichosporium OB3b MMOH (gray, PDB ID: 1MHY) (20) and the M. sporium strain 5 MMOH-MMOD complex (yellow). The two gate-forming residues (Leu110 and Phe188) are shown as red spheres, and 1,6-hexanediol is shown in green. The cavities calculated using PyMOL are shown as surface models. (C) Schematic model showing the association of MMOH with its auxiliary proteins MMOB and MMOD, which compete for the canyon region of MMOH. Hanseong Kim et al. Sci Adv 2019;5:eaax0059 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).