- In Clinic Titer Testing for Core Vaccines

- An Overview

Biogal Galed Labs. Experience -established in 1986. •A leader in veterinary diagnostic kits. •Serologic and direct evaluation of veterinary diseases. •Customers > 50 countries.

Biogal’s Markets > 50 distributors worldwide and 250 endusers directly

Biogal - Extensive Range of Unique Veterinary Diagnostic Solutions: Molecular diagnostic, portable, fast, in clinic/lab Validate Before You Vaccinate Portable, Compact, Fast Semi Quantitative ELISA

Biogal – Kits Available: Canine (ICH-CPV-CDV) Feline (FPLV-FHV-FCV) Canine Parvo & Distemper IgM Canine Ehrlichia canis Canine Leptospira Feline T.gondi & C.psittaci Feline Corona Virus (FIP) Avian Chlamydophila psittaci Canine Brucella Leptospira Ehrlichia canis Leishmania infantum Anaplasma platys Babesia canis Babesia gibsoni Canine/Feline parvovirus Mycoplasma haemofelis FeLV DNA soon – Distemper RNA

- About VacciCheck®

A Diagnostic Tool VacciCheck®: Semi Quantitative In Clinic Titer Test Measures antibody levels in blood or serum Based on unique ImmunoComb principle - solid-phase ELISA

Antibody Test Kit - Contents Developing Plate Comb Tweezers Comb Scale Pipette

Main Components The developing plate contains the reagents needed for Comb development. Antigens are attached to each tooth of the Comb. The placement of the antigens is shown by the labeling on the Comb.

The color intensity of spots corresponds to antibody level. Analyzing Results Example of a Developed Comb for testing Feline Panleukopenia, Calici and Herpes Virus The color intensity of spots corresponds to antibody level. Control Panleukopenia Herpes Calici

Scoring Results qualitative results Reading results by eye semi-quantitative results Reading results using CombScale quantitative results Reading results using CombScan

As appears in Vaccination Guidelines In Clinic Titer Testing for Core Vaccines As appears in Vaccination Guidelines

VacciCheck – Recommended by WSAVA Source: WSAVA Vaccination Guidelines 2016 “Since publication of the 2010 guidelines there have been advances in the availability of rapid and simple in-practice serological test kits that can detect the presence of protective antibody specific for CDV, CAV and CPV-2 in individual dogs……. Two commercially produced test kits are available and have been applied and validated in the practice and shelter setting (Gray et al. 2012, Litster et al. 2012) [EB1]. These test kits have proven popular with veterinarians who wish to be able to offer their clients an alternative to routine core revaccination at 3-yearly intervals,” 

Recommended by AAHA and WSAVA VACCINATION GUIDELINES GROUPS for: • Determining whether a puppy/kitten has responded to vaccination. • Testing of newly adopted adult dogs/cats of unknown vaccination history. • VacciCheck INSTEAD of automatic triennial core revaccination for adult dogs. • Management of outbreaks in shelters.

Puppy Titer Testing Source: WSAVA Vaccination Guidelines 2016

Critical Q & A for Serology Core Vaccine Testing Q&A: 85. In an animal that has completed its puppy/kitten shots, is a higher antibody titre required to protect against heavy disease challenge? For CDV, CAV-2, CPV-2 and FPV the answer is no. The presence of antibody (no matter what the titre) indicates protective immunity and immunological memory is present in that animal. Giving more frequent vaccines to animals in an attempt to increase antibody titre is a pointless exercise. It is impossible to create ‘greater immunity’ by attempting to increase an antibody titre. Q&A: 86. Can we test dogs as an alternative to annual vaccination? We are concerned about the advice to only boost every 3 years. Yes, certainly. There are now well-validated in-practice serological test kits that permit determination of the presence of protective serum antibody specific for CDV, CAV, CPV-2 and FPV. In other countries, these kits are used to confirm protection at 3-yearly intervals (instead of automatic revaccination for core diseases). You could perform serology annually, but if you were to collect and analyze the data that you generated within your practice, you will quickly find that annual testing is unjustified. Source: Editorial WSAVA Vaccination Guidelines 2016

2 of the 7 Indications for Serology Testing:

To assess the immunity when there is a prior vaccination history in an adult dog but the dog is due (or is overdue) for a scheduled booster.

To assess the immunity following completion of the initial vaccination series in puppies >24 wk of age

Key VacciCheck Clinical Papers WSAVA recommendations for VacciCheck: Read full article  AAHA recommendations for VacciCheck: Read full article Professor Richard Ford Antibody Titers versus Vaccination Read full article Professor Michael J. Day VacciCheck Used in a Major Clinical Study Read  

In the Press One way to insure comfort for pet owners and veterinarians alike is to titer test dogs every three years. A titer test checks to determine the level of protective antibodies. If those antibodies exist, why revaccinate? Schultz asks. A new titer test, called Canine VacciCheck, just now being made available to veterinarians, is faster and easier, Schultz notes.