Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells. Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells.

Slides:

Advertisements

Similar presentations

T3 increased J7-TRα1 cell migration and proliferation both in vitro and in vivo. T3 increased J7-TRα1 cell migration and proliferation both in vitro and.

Advertisements

Histopathologic features (all images at ×10 magnification).

Effect of vascular endothelial growth factor and its receptor KDR on the transendothelial migration and local trafficking of human T cells in vitro and.

25(OH)D and 1,25(OH)2D perfusion treatment effect on cancer-related markers: IF stains illustrating expression levels for Ki-67 (A), cyclin D1 (B), ErbB-2.

Sunitinib plus rMVA–CEA–TRICOM vaccine decreased tumor burden and increased intratumoral infiltration of T lymphocytes in the MC38-CEA colon carcinoma.

IHC staining of FFPE esophageal tissues in tissue microarrays (×20).

CaMKII inhibition in human primary and pluripotent stem cell-derived chondrocytes modulates effects of TGFβ and BMP through SMAD signaling B. Saitta,

LATS2 is a tumor suppressor in a mouse lumB breast cancer model.

Inhibition of KLF4 by Statins Reverses Adriamycin-Induced Metastasis and Cancer Stemness in Osteosarcoma Cells Yangling Li, Miao Xian, Bo Yang, Meidan.

Volume 20, Issue 5, Pages e7 (May 2017)

by Silvia Mele, Stephen Devereux, Andrea G

Cardiac Fibrosis at 18 Weeks Post TAC (A) Representative images (original magnification ×20) of Picrosirius red-stained cardiac tissue sections from HF +

Fig. 4 TNF-α up-regulates Fas/Fap-1 expression to promote IL-1RA–sEV release in murine MSCs. TNF-α up-regulates Fas/Fap-1 expression to promote IL-1RA–sEV.

2aG4 directly induces monocytic MDSCs to differentiate into dendritic cells and macrophages by binding to phosphatidylserine on their cell surface. 2aG4.

Effect of antiangiogenic TKIs and rMVA–CEA–TRICOM vaccine on tumor compactness, tight junctions, and intratumoral pressure in the MC38-CEA model. Effect.

Quantification of MHC-I, β2m, and T-cell subsets.

Murine gingival MSCs and skin MSCs produce and secrete IL-1RA–EV

Cbx4 Sumoylates Prdm16 to Regulate Adipose Tissue Thermogenesis

Effect of insulin on hepcidin expression in HepG2 cells.

SIRT1 is downregulated in human gastric cancer (GC).

CPI-444 efficacy requires CD8+ T cells and is associated with increased CD73 expression. CPI-444 efficacy requires CD8+ T cells and is associated with.

CYP1A1 knockdown increases cell death.

Gene expression changes associated with response to combination CPI-444 and anti–PD-L1 treatment in MC38 tumors. Gene expression changes associated with.

TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor tissues. TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor.

Effect of sustained suppression of HDAC2 in MKN-1 cells on in vitro tumorigenicity. Effect of sustained suppression of HDAC2 in MKN-1 cells on in vitro.

Variation in dUTPase expression in cell lines.

Geographic colocalization of PD-L1+ tumor cells with infiltrating immune cells in MCC. The predominant pattern of tumor cell PD-L1 expression is at the.

IL32 is highly expressed in mycosis fungoides lesional skin.

Effects of DPM treatment on tumor volume, tumor mass, and pulmonary metastasis at experimental end point. Effects of DPM treatment on tumor volume, tumor.

LINC00092 binds with PFKFB2 to promote ovarian cancer metastasis.

AXL is not expressed in human prostate tumors.

A, one-step viral growth curves: RT treatment increased the virus yield in MV-CEA–treated U87 cells by up to 2 log as compared with MV-CEA infection only.

Expression of CRC stem cell markers and L1 in CRC cells.

Efficient and preferential pairing of cysteine-modified TCRs

In vivo tumorigenicity of MKN-1 cells with sustained suppression of HDAC2. In vivo tumorigenicity of MKN-1 cells with sustained suppression of HDAC2. A,

RNF2 promotes TGFβ signaling.

CDN–treated MOC1 tumors demonstrate enhanced activation of innate and antigen-specific adaptive immunity. CDN–treated MOC1 tumors demonstrate enhanced.

DNA demethylation reactivates GSN expression in gastric cancer cells.

Intratumoral lymphoid aggregates are sites of postvaccination T-cell activation and regulation. Intratumoral lymphoid aggregates are sites of postvaccination.

Effects of HDAC2 inactivation on the invasive potential of human gastric cancer cells. Effects of HDAC2 inactivation on the invasive potential of human.

Reduced klotho expression in pancreatic cancer.

FBXL19-AS1 knockdown inhibits proliferation, migration and invasion, and reduces the expression levels of angiogenesis related proteins in lung cancer.

Effect of CDV on human SF7796 xenografts in vivo.

Effect of SPINDLIN1 protein on cancer cell proliferation and invasion.

MAPK/ERK signaling regulates TLR4 gene expression in response to BRAFV600E. MAPK/ERK signaling regulates TLR4 gene expression in response to BRAFV600E.

Quantitative PTEN protein expression is associated with pertuzumab sensitivity in vitro and trastuzumab resistance in vivo. Quantitative PTEN protein expression.

Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Expression.

Γ-TmT reduces serum E2 levels and induces estrogen-metabolizing enzyme CYP1A1 in E2-treated ACI rats. γ-TmT reduces serum E2 levels and induces estrogen-metabolizing.

Anti-CD40 activates TAMs and recruits inflammatory monocytes.

PVT1 binds to the FOXM1 protein and increases its protein level by enhancing its stability. PVT1 binds to the FOXM1 protein and increases its protein level.

SAHA blocks IR-induced increase of RAD51 protein in MM cells.

Combination of R848 and anti-CD200R affects activation of tumor-infiltrating myeloid cells. Combination of R848 and anti-CD200R affects activation of tumor-infiltrating.

Targeting GAPLINC decreased CD44 expression and tumor growth in vivo.

HOXA11-AS acts as a ceRNA for miR-1297.

TGFβ1/Smad3 signaling suppresses IFNγ production via an E4BP4-dependent mechanism in NK-92 cells. TGFβ1/Smad3 signaling suppresses IFNγ production via.

PARP1 suppresses the transcription of PD-L1 in cancer cells.

Effect of dexamethasone on PCDGF/GP88 mRNA and protein expressions and effect of PCDGF/GP88 on dexamethasone-induced cell death. Effect of dexamethasone.

Influence of minocycline on TGF-β1 expression and TGF-β1–induced NF-кB activation in ovarian cancer cells. Influence of minocycline on TGF-β1 expression.

G34 H3K36me3 upregulates MYCN which is selectively targetable by kinases that destabilize the protein. G34 H3K36me3 upregulates MYCN which is selectively.

Correlations between APOBEC expression and immune cell markers across 22 cancer types. Correlations between APOBEC expression and immune cell markers across.

Human melanoma induces paracrine β-catenin signaling activation in DCs in vitro. Human melanoma induces paracrine β-catenin signaling activation in DCs.

Recruitment of CD8+, CD4+, and Foxp3+ cells into oral lesions in response to anti–PD-1 treatment. Recruitment of CD8+, CD4+, and Foxp3+ cells into oral.

Tobacco-specific carcinogens induce methylation, DNA adducts, DNA damage, and activation of DNMT1. Tobacco-specific carcinogens induce methylation, DNA.

TAMs upregulate DNMT1 in gastric cancer cells through the CCL5/CCR5/STAT3 pathway. TAMs upregulate DNMT1 in gastric cancer cells through the CCL5/CCR5/STAT3.

EC-derived SP cells are targeted by CD30.CAR T cells.

Hoxa9 is partly required for Twist1-induced migration, invasion, anoikis resistance, and soft agar tumorigenicity of Myc-CaP cells. Hoxa9 is partly required.

Coincidence and prognostic significance of PD-1+ and CD103+ cells in HGSC. Serial sections from the 490-case TMA were stained with antibodies to CD103.

Silencing E4BP4 abrogates the promoting effect of SMAD3 knockdown on IFNγ production in NK-92 cells. Silencing E4BP4 abrogates the promoting effect of.

IHC staining of FFPE esophageal tissues in tissue microarrays (×20).

GSN and DNMT1 expression are inversely correlated in a pattern associated with patient survival. GSN and DNMT1 expression are inversely correlated in a.

Presentation transcript:

Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells. Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells. A, The M2 marker CD204 (left) and the M1 marker CD86 (right) expression in early- and advanced-stage gastric cancer was analyzed by IHC staining. Magnification: 100× (top). Images on the bottom panel are high-magnification (400×) of areas outlined by black squares. B, IF staining of CD204 (green) and DNMT1 (red) was performed in early-stage and advanced-stage gastric tumors. Magnification: 200× (top). Images on the bottom panel are high magnification (400×) of areas outlined by white squares. C, After coculture with U937 cells, protein and mRNA levels of DNMT1 and GSN in AGS and HR cells were analyzed by Western blotting and qPCR, respectively. Fold changes in protein and mRNA expression were calculated relative to gastric cancer cell monoculture. *, P < 0.05; **, P < 0.01 versus monocultures, two-way ANOVA. D, DNMT1 and GSN protein expression in AGSshLuc and AGSshDNMT1 cells cocultured with U937 cells was analyzed by Western blotting. Fold changes in protein expression was determined relative to monoculture of AGSshLuc cells. E, The motility of AGSshLuc and AGSshDNMT1 cells with or without U937 coculture was determined by Transwell migration assay. The bar graph represents the fold changes in the number of migrating cells relative to AGSshLuc monoculture. **, P < 0.01; ***, P < 0.001, significant differences between groups, two-way ANOVA. F, AGS cells in monoculture or cocultured with U937 cells were treated with PCA. DNMT1 activity was determined by the DNMT activity assay. GSN expression was detected by Western blotting. **, P < 0.01; ***, P < 0.001, significant differences between groups, two-way ANOVA. Hao-Chen Wang et al. Cancer Immunol Res 2017;5:885-897 ©2017 by American Association for Cancer Research