Fig. 4 Cytosolic delivery of native enzymes.

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Fig. 4 Cytosolic delivery of native enzymes. Cytosolic delivery of native enzymes. (A) β-Gal catalyzes the hydrolysis of colorless substrate X-Gal into a blue-colored product. (B) X-Gal staining of the cells treated with β-Gal/P4 for 4 hours. (C) Enzymatic activity of the β-Gal after cytosolic delivery determined by a kit. The dose of β-Gal in each well was 5 μg. (D) HRP catalyzes colorless substrates Amplex Red or TMB into a red fluorescent product, resorufin, or a blue dye, respectively, in the presence of hydrogen peroxide. (E) Confocal microscopy images of HeLa cells treated with P4/HRP and Amplex Red. Commercial reagents PULSin and TransEx were used as positive controls. (F) HRP enzymatic activity in the transduced cell analysis by TMB assay. The dose of HRP in each well was 6 μg. The dose of P4 in the experiments was 8 μg in each well. Chongyi Liu et al. Sci Adv 2019;5:eaaw8922 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).