TbERP1, TbERP2, TbERP3, and TbERP8 are codependent for stability.

Slides:

Advertisements

Similar presentations

Control siRNA BRG1 siRNA PTEN – Akt – p-Akt – p-GSK-3  – Cyclin D1 – BRG1 –  -actin – SW480 Supplemental data S1 Supplemental data S1. Effects of transduction.

Advertisements

Volume 6, Issue 5, Pages (November 2004)

Inhibition of glycogen synthase kinase-3 activity leads to epigenetic silencing of nuclear factor κB target genes and induction of apoptosis in chronic.

Volume 76, Issue 1, Pages (July 2009)

Expression and regulation of MD-2s.

NRF2 Is a Major Target of ARF in p53-Independent Tumor Suppression

Production of recombinant KRBV NS1 and antiserum reactivity.

Cellular localization of the chimeric Iff5-Iff1C, Iff5-Iff2C, Iff5-Iff4C, Iff5-Iff7C, and Iff5-Iff10C proteins. Cellular localization of the chimeric Iff5-Iff1C,

Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ mpk1Δ, and khd1Δ ccr4Δ mpk1Δ mutant strains. Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ.

Lipotransin Molecular Cell

Esther B.E. Becker, Azad Bonni Neuron

Posttranscriptional Crossregulation between Drosha and DGCR8

A, Western blot analysis of fetuin-A in AGA (lanes 1–5 and 10–13) and IUGR (lanes 6–9, 14, and 15) UC plasma samples. A, Western blot analysis of fetuin-A.

Truncated protein isoforms of CD19 provide proliferative advantage.

Rpn11p mutation affects the recruitment of some other host factors into the tombusvirus replicase in yeast. Rpn11p mutation affects the recruitment of.

Effect of small TbTim knockdown on cell growth.

Cellular localization of the Iff2, Iff3 (or -9), Iff5, Iff6, and Iff8 proteins. Cellular localization of the Iff2, Iff3 (or -9), Iff5, Iff6, and Iff8 proteins.

UUKV NSs protein inhibits IFN-β induction at the level of MAVS, whereas HRTV NSs antagonizes at the level of TBK1. UUKV NSs protein inhibits IFN-β induction.

Volume 61, Issue 6, Pages (June 2002)

Localization of the Iff8 extended protein.

Temporal Regulation of Salmonella Virulence Effector Function by Proteasome- Dependent Protein Degradation Tomoko Kubori, Jorge E. Galán Cell Volume.

Volume 26, Issue 6, Pages (June 2007)

Silva H Hanissian, Raif S Geha Immunity

Overexpression of BTAK/Aurora-A in ovarian carcinoma.

Supplementary Figure 1 A B C SW620 HT29 SW620

Immunoblot analysis of GpGCS1 expression in strains K41 (mating-type plus) and K34 (mating-type minus). Immunoblot analysis of GpGCS1 expression in strains.

Stress-protective roles of C. auris Hog1.

Knockdown efficiency of different shRNAs.

Deletion of the multicopy A2 family genes and increased efficiency through coselection for parasites with CRISPR-Cas9 activity. Deletion of the multicopy.

TGF-β stimulates SMAD7 expression via FOXO3.

Abraxas binds to BRCT–PALB2(L21P) and is involved in the recruitment of this fusion protein or of endogenous PALB2 to sites of DNA damage. Abraxas binds.

Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant.

Wild-type NSD3 is required for the blockade of differentiation in BRD4–NUT-expressing NMC cells. Wild-type NSD3 is required for the blockade of differentiation.

Stability of SsbA (A) and GFP (B) proteins in UA159 and ΔclpX strains.

Synergistic WNT1 and WNT7B signaling is downstream of LRP6 phosphorylation and β-catenin stabilization. Synergistic WNT1 and WNT7B signaling is downstream.

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

CML-HSA treatment activates MAPK family members ERK1/2 and p38 but not JNK. Samples were taken at the indicated times after 100 μg/ml CML-HSA exposure.

PER is a substrate of CK1α.

SusG LES is required for efficient packing into OMVs

TbERP silencing has minimal effect on proliferation.

BCL-3 regulates expression of stemness-associated Wnt targets.

p65 depletion increases basal protein aggregation and insolubilization

TbERP1, TbERP2, TbERP4, and TbERP8 localize to ERES in PCF cells.

Low pH alters Ras1 localization and its activation state, but these changes are not responsible for the effect of pH on morphology. Low pH alters Ras1.

Construction and analysis of C. auris hog1Δ cells.

A, IGFBP-3 knockdown via siRNA using real-time qRT-PCR analysis.

BORIS mRNA expression in DNMT deficient HCT116 cell lines.

Induction of CDCP1 is regulated by Ras in NSCLC cells.

BME treatment increases granzyme B expression in NK 3.3 cells.

Phosphorylation of TET2 by JAK2 in response to hematopoietic cytokines

Curcumin suppresses the expression of antiapoptotic proteins in multiple myeloma cells. Curcumin suppresses the expression of antiapoptotic proteins in.

A. A. Honokiol inhibits TNF-induced NF-κB activation, IκBα phosphorylation, and IκBα degradation. Honokiol inhibits TNF-induced activation of NF-κB. H1299.

Gain-of-function conferred by RNF31 SNPs

TET2 interacts with KLF1 transcription factor.

Secretion of TNF-α from macrophages and monocytic cells were reduced by the deletion of Nrdc. Secretion of TNF-α from macrophages and monocytic cells were.

Expression of dominant-negative RasN17 completely suppresses Ras activation in Rh1 cells. Expression of dominant-negative RasN17 completely suppresses.

Fig. 1 Dcr-2 is required for Toll signaling in Drosophila S2 cells.

ODC protein expression in paired benign and cancer tissue obtained from patients with PCA. The protein expression was measured by immunoblot analysis in.

Validation of OMV- and OM-enriched proteins.

SAHA blocks IR-induced increase of RAD51 protein in MM cells.

Fig. 4 Dcr-2 binds to the 3′UTR of Toll mRNA.

GlcN-induced knockdown of PfHsp70x does not affect intraerythrocytic growth. GlcN-induced knockdown of PfHsp70x does not affect intraerythrocytic growth.

Induction of PARP cleavage (A) and activation of caspases (B) after treatment with a combination of TRAIL and cisplatin. Induction of PARP cleavage (A)

Fig. 5 Dcr-2 directly binds to Toll 3′UTR.

Evidence for diverse lysine-linked ubiquitin-modified proteins in T

TbERP4 mediates GPI-dependent trafficking in PCF cells.

A B C Name Sequence TIMP3 promoter

CREB1 binds at the proximal region of the TGFB2 promoter and induces its transcriptional activation. CREB1 binds at the proximal region of the TGFB2 promoter.

TCTP enhances the protein stability of Pim-3 by blocking the ubiquitin–proteasome degradation of Pim-3. TCTP enhances the protein stability of Pim-3 by.

Biosynthetic trafficking of TbCatL is TbERP dependent.

Presentation transcript:

TbERP1, TbERP2, TbERP3, and TbERP8 are codependent for stability. TbERP1, TbERP2, TbERP3, and TbERP8 are codependent for stability. The in situ TbERP epitope-tagging constructs were transfected into each of the TbERP RNAi cell lines. Specific dsRNA was induced for 72 h, and whole-cell lysates were prepared from control (Tet-) and induced (Tet+) cells, as indicated in the column labels. Lysates were fractionated by SDS-PAGE (1 × 107 cell equivalents per lane). Membranes were first immunoblotted with anti-TY or anti-HA to evaluate TbERP protein stability under RNAi, as indicated in the row labels. Blots were then stripped and reprobed for cytosolic Hsp70 as a loading control. Two biological replicates are presented (total n = 3). qRT-PCR analyses confirmed that RNAi knockdown is specific, and mRNA levels of opposing tagged TbERP constructs were unaffected, even under significant protein depletion (not shown). Emilia K. Kruzel et al. mSphere 2017; doi:10.1128/mSphere.00282-17