SDS-PAGE and Coomassie Blue Staining of Protein Samples after Various Purification Steps of Both Rice and Barley ADPG Hydrolytic NPPs.Lane numbering of.

Slides:



Advertisements
Similar presentations
Supplementary Table 1. Purification of Chi-V from E. coli Step Protein Activity Specific activity Yield (mg) (mmol/min) (mmol/min/mg) (%) Crude extract.
Advertisements

1&3: hGH-sc(Fc)2 2&4: hGH-Fc anti-Fc anti-hGH
Effects of Growth Factors and Basement Membrane Proteins on the Phenotype of U- 373 MG Glioblastoma Cells as Determined by the Expression of Intermediate.
Fig. 4. Expressional profiles of apolipoproteins in HDL
Target protein Additional file 3. SDS-PAGE showing the degree of purification of D1-26PtxtPL1-27 expressed in E. coli. PtxtPL1-27.
M M Additional file 2 - Silver staining of protein extract and tryptic digest run on SDS-PAGE. The figure shows.
Comparison of a tetravalent single-chain antibody-streptavidin fusion protein and an antibody-streptavidin chemical conjugate for pretargeted anti-CD20.
Phylogenetic analysis of CADs and oxidoreductases involved in specialized metabolism (ORSMs). Phylogenetic analysis of CADs and oxidoreductases involved.
Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,
Interaction of SRP19 with nuclear transport receptors.
by Kazuhiko Adachi, Takamasa Yamaguchi, Jian Pang, and Saul Surrey
Quantification of intracellular fuzzy staining (A) and of spherical bodies (B) in root cells upon an NaCl treatment. Quantification of intracellular fuzzy.
Gloria García-Casado, PhD, Jesús F
Dual-Luciferase Activation Assay with Potential Targets of ZmbZIP22.
Fast protein LC IMAC purification of cytosolic phosphoproteins
H-NS2 protein interacts with Lon and H-NS protein in vitro.
Extension of the GlyRβ binding site on gephyrin.
A Proteolytic Cascade of Kallikreins in the Stratum Corneum
TEV protease elution from cellulose is specific and provides highly purified target protein. TEV protease elution from cellulose is specific and provides.
SDS–PAGE and immunoblots of purified IgG for citrullination and carbamylation. a) Representative Coomassie-blue-stained isolated total IgG from patients.
Transport receptors bind directly to SRP19 in a RanGTP-regulated manner. Transport receptors bind directly to SRP19 in a RanGTP-regulated manner. A GST-SRP19.
Percentage of proteins identified in envelope membrane extracts according to the purification method and the number of transmembrane domains. Percentage.
Differential conjugation of endogenous SUMO-1 and endogenous SUMO-2/3 to target proteins.A and B, SUMO-1 and SUMO-2 proteins were produced in E coli and.
MAPKKK5-induced cell death is independent of Ser-289 phosphorylation.
Glc uptake in roots and gene expression in barley seedlings.
Bottom-up proteomic characterization of MALDI IMS samples.
Mass spectrometry identification of spots in 2D blue native gels of cytoplasmic membranes isolated from BL21(DE3)pLysS Spots in 2D BN gels of cytoplasmic.
NanoLC-MS/MS/based analysis of proteome differences between colonospheres and isogenic differentiated tumor cells. NanoLC-MS/MS/based analysis of proteome.
Proteins previously reported in published MALDI IMS studies and their frequency of observation in the present study. Proteins previously reported in published.
Characterization of aggregates isolated from E
Representative 2-D gel images of UC plasma from AGA (A) and IUGR (B) neonates are shown. Representative 2-D gel images of UC plasma from AGA (A) and IUGR.
Lnp protein requires phospholipids to form stacked discs.
Analysis of whole cell lysates by Western blotting and 2D gel electrophoresis.A, cells overexpressing GFP fusion proteins and control cells were cultured.
Extraction of proteins from MALDI IMS slides.
Preferential conjugation of proteins to SUMO-1 or SUMO-2
2-D gel images visualized by Coomassie Brilliant Blue staining representing total proteins extracted from HCT-8 under apoptotic conditions in 2 mm Gln.
Enlargements of 2-D gels visualized by Coomassie Brilliant Blue staining. Enlargements of 2-D gels visualized by Coomassie Brilliant Blue staining. In.
SDS-PAGE of IGFBP-5 from 32P-labeled T47D cells and separation of tryptic phosphopeptides separated by HPLC.a, an autoradiograph (lane 1) is shown next.
Purity of Lnp and its derivatives.
Chlorophyll concentration (conc
Western blot analysis of histone H1.X.
Fig. 4. Coilin phosphomutant displays differential RNA binding and degradation activities.Purified proteins were analyzed for RNase activity with total.
The Zn2+-finger domain is not essential for the formation of stacked bicelles. The Zn2+-finger domain is not essential for the formation of stacked bicelles.
Full-length TAPL interacts specifically with LAMP-1 and LAMP-2.
Interaction of NF-L with C1 cassette-containing NR1 splice variants.
A: Representative Western blot showing a band migrating at ∼90 kDa corresponding to neprilysin protein. A: Representative Western blot showing a band migrating.
Active MT1-MMP is recycled to the cell surface in HT1080 cells.
WW domain does not show dominant-negative effect on TBSV replication in a yeast cell extract. WW domain does not show dominant-negative effect on TBSV.
Characterization of SsPV1/WF-1 isolated from hypovirulent strain WF-1.
Key functional sites of SPINDLIN1 could be phosphorylated by Aurora-A.
Mapping the Pirh2 and p73 interaction sites.
MTB Proteins Lack a Canonical MID and Act as Transcriptional Repressors. MTB Proteins Lack a Canonical MID and Act as Transcriptional Repressors. (A) Y2H.
Predicted Amino Acid Sequence of the Tomato Cf-4 Protein (Thomas et al
Intracellular Immunolocalization of GFP in Either GFP- or NPP1-GFP–Expressing Transgenic Rice Cells and Untransformed Rice Cells.(A) GFP-expressing transgenic.
Effect of SFN on the protein expression of Nrf2, HO-1, and NQO1 in JB6-shMock and JB6-shNrf2 cells. Effect of SFN on the protein expression of Nrf2, HO-1,
Western blot analysis of plasma proteins from two-dimensional gel electrophoresis. Western blot analysis of plasma proteins from two-dimensional gel electrophoresis.
2D-PAGE gels showing differentially expressed proteins in Panc-1 cells transfected with siNR4A1 and functional classification of the proteins. 2D-PAGE.
DNA-binding and double-strand break formation by Vpr.
Dcr-2 directly interacts with Toll 3′UTR via its PAZ domain in vitro
Fig. 5 Dcr-2 directly binds to Toll 3′UTR.
SDS-PAGE and Immunoblot Analyses of Cucurbita PP1 and PP2 in Phloem Exudates of 13-Day-Old Intergeneric Grafts of Cucumiss ativus on Cucurbita maxima.(A)
Fig. 3 TBK1 phosphorylates RAB7AS72 but not the equivalent residue in other RABs in vitro. TBK1 phosphorylates RAB7AS72 but not the equivalent residue.
Transformation of lsf1 Plants with LSF1 Variants.
Rapamycin-Dependent Interaction between TOR-FRB Domain and Hs FKBP12
EAR1 Represses ABA Signaling.
RRC1 Interacts with phyB and Colocalizes in Nuclear Photobodies.
MED25 and JAZ7 Compete to Interact with MYC2.
Clade 2 ERFs Differentially Bind and Activate a PMT2 Promoter
Alignment of the Amino Acid Sequences of NCS and Other PR10/Bet v1 Proteins from Various Plant Species.Deduced amino acid sequences were aligned using.
MTB1 Interacts with MYC2 and Disrupts MED25-MYC2 Interaction.
Presentation transcript:

SDS-PAGE and Coomassie Blue Staining of Protein Samples after Various Purification Steps of Both Rice and Barley ADPG Hydrolytic NPPs.Lane numbering of barley NPP purification corresponds to Table 1. SDS-PAGE and Coomassie Blue Staining of Protein Samples after Various Purification Steps of Both Rice and Barley ADPG Hydrolytic NPPs.Lane numbering of barley NPP purification corresponds to Table 1. Lane numbering of rice NPP purification corresponds to (1) crude extract, (2) acid pH treatment, and (3) purified enzyme (see Table 2). Yohei Nanjo et al. Plant Cell 2006;18:2582-2592 ©2006 by American Society of Plant Biologists