B7-H3 Associated with Tumor Progression and Epigenetic Regulatory Activity in Cutaneous Melanoma  Jinhua Wang, Kelly K. Chong, Yoshitaka Nakamura, Linhda.

Slides:



Advertisements
Similar presentations
Volume 342, Issue 1, Pages (January 2014)
Advertisements

Endosialin (Tem1) Is a Marker of Tumor-Associated Myofibroblasts and Tumor Vessel- Associated Mural Cells  Sven Christian, Renate Winkler, Iris Helfrich,
Stefanie Krenzer, Heike Peterziel, Cornelia Mauch, Sachiko I
MicroRNA-21 Expression in CD4+ T Cells Is Regulated by STAT3 and Is Pathologically Involved in Sézary Syndrome  Leslie van der Fits, Marloes S. van Kester,
Cell Surface CD74–MIF Interactions Drive Melanoma Survival in Response to Interferon-γ  Keiji Tanese, Yuuri Hashimoto, Zuzana Berkova, Yuling Wang, Felipe.
Volume 145, Issue 4, Pages e9 (October 2013)
A Signal Transduction Pathway from TGF-β1 to SKP2 via Akt1 and c-Myc and its Correlation with Progression in Human Melanoma  Xuan Qu, Liangliang Shen,
IMP-3 Promotes Migration and Invasion of Melanoma Cells by Modulating the Expression of HMGA2 and Predicts Poor Prognosis in Melanoma  Yi-Shuan Sheen,
Matias A. Bustos, Shigeshi Ono, Diego M
Jasmine George, Minakshi Nihal, Chandra K
MicroRNA-31 Promotes Skin Wound Healing by Enhancing Keratinocyte Proliferation and Migration  Dongqing Li, X.I. Li, Aoxue Wang, Florian Meisgen, Andor.
MicroRNA-489 Plays an Anti-Metastatic Role in Human Hepatocellular Carcinoma by Targeting Matrix Metalloproteinase-7  Yixiong Lin, Jianjun Liu, Yuqi Huang,
Plasma MicroRNA-21 Is Associated with Tumor Burden in Cutaneous Melanoma  Gerald Saldanha, Linda Potter, Priya Shendge, Joy Osborne, Steve Nicholson, NgiWieh.
Psoriasis Skin Inflammation-Induced microRNA-26b Targets NCEH1 in Underlying Subcutaneous Adipose Tissue  Louisa Cheung, Rachel M. Fisher, Natalia Kuzmina,
Cleidson P. Alves, Milene H. Moraes, Josane F. Sousa, Carmen Lucia S
Inhibition of SOX17 by MicroRNA 141 and Methylation Activates the WNT Signaling Pathway in Esophageal Cancer  Yan Jia, Yunsheng Yang, Qimin Zhan, Malcolm.
Verena N. Lorenz, Michael P. Schön, Cornelia S. Seitz 
Complement Factor H: A Biomarker for Progression of Cutaneous Squamous Cell Carcinoma  Pilvi M. Riihilä, Liisa M. Nissinen, Risto Ala-aho, Markku Kallajoki,
Decreased Expression of the Chromatin Remodeler ATRX Associates with Melanoma Progression  Zulekha A. Qadeer, Sara Harcharik, David Valle-Garcia, Chen.
Zhuo Li, Dieter Metze, Dorothea Nashan, Carsten Müller-Tidow, Hubert L
Epidermal Growth Factor Facilitates Melanoma Lymph Node Metastasis by Influencing Tumor Lymphangiogenesis  Andreas Bracher, Ana Soler Cardona, Stefanie.
CCN2 Expression by Tumor Stroma Is Required for Melanoma Metastasis
Glyoxalase I Is Differentially Expressed in Cutaneous Neoplasms and Contributes to the Progression of Squamous Cell Carcinoma  Xiao-Yan Zou, Dong Ding,
Melanoma Cell Invasiveness Is Promoted at Least in Part by the Epidermal Growth Factor–Like Repeats of Tenascin-C  Jelena Grahovac, Dorothea Becker, Alan.
EIF4E Is an Adverse Prognostic Marker of Melanoma Patient Survival by Increasing Melanoma Cell Invasion  Shahram Khosravi, Kevin J. Tam, Gholamreza S.
Tie2-R849W Mutant in Venous Malformations Chronically Activates a Functional STAT1 to Modulate Gene Expression  Hsiao-Tang Hu, Yi-Hsien Huang, Yi-Ann.
Thyroid Transcription Factor-1 Amplification and Expressions in Lung Adenocarcinoma Tissues and Pleural Effusions Predict Patient Survival and Prognosis 
MiR-137 Inhibits the Invasion of Melanoma Cells through Downregulation of Multiple Oncogenic Target Genes  Chonglin Luo, Paul W. Tetteh, Patrick R. Merz,
Rolando Perez-Lorenzo, Kamraan Z. Gill, Che-Hung Shen, Feng X
The Hematopoietic Stem Cell Regulatory Gene Latexin Has Tumor-Suppressive Properties in Malignant Melanoma  Viswanathan Muthusamy, Sanjay Premi, Cara.
Osteopontin Expression Correlates with Melanoma Invasion
Molecular Therapy - Nucleic Acids
Leon Raskin, Douglas R. Fullen, Thomas J. Giordano, Dafydd G
MicroRNA-101 Exerts Tumor-Suppressive Functions in Non-small Cell Lung Cancer through Directly Targeting Enhancer of Zeste Homolog 2  Ji-guang Zhang,
SOX4 Promotes Proliferative Signals by Regulating Glycolysis through AKT Activation in Melanoma Cells  Wei Dai, Xinyuan Xu, Shuli Li, Jingjing Ma, Qiong.
Pro-Invasive Activity of the Hippo Pathway Effectors YAP and TAZ in Cutaneous Melanoma  Flore Nallet-Staub, Véronique Marsaud, Ling Li, Cristèle Gilbert,
Serum miR-16: A Potential Biomarker for Predicting Melanoma Prognosis
RANK Is Expressed in Metastatic Melanoma and Highly Upregulated on Melanoma- Initiating Cells  Verena Kupas, Carsten Weishaupt, Dorothee Siepmann, Maria-Laura.
Activin A Is Anti-Lymphangiogenic in a Melanoma Mouse Model
Absence of Distinguishing Senescence Traits in Human Melanocytic Nevi
Jinhua Wang, Sharon K. Huang, Diego M. Marzese, Sandy C. Hsu, Neal P
SIRT1 Activation Ameliorates Aldara-Induced Psoriasiform Phenotype and Histology in Mice  Sijing Xie, Zhonglan Su, Bin Zhang, Jiuyu Ge, Shiyu Song, Guibo.
Discoidin Domain Receptor 2–microRNA 196a–Mediated Negative Feedback against Excess Type I Collagen Expression Is Impaired in Scleroderma Dermal Fibroblasts 
Human Mitochondrial NAD(P)+–Dependent Malic Enzyme Participates in Cutaneous Melanoma Progression and Invasion  Yung-Lung Chang, Hong-Wei Gao, Chien-Ping.
MiR-125b, a MicroRNA Downregulated in Psoriasis, Modulates Keratinocyte Proliferation by Targeting FGFR2  Ning Xu, Petter Brodin, Tianling Wei, Florian.
Decreased Intraindividual HLA Class I Expression is due to Reduced Transcription in Advanced Melanoma and Does Not Correlate with HLA-G Expression  Jörg.
Yabin Cheng, Guangdi Chen, Magdalena Martinka, Vincent Ho, Gang Li 
Epigenetic Silencing of SPINT2 Promotes Cancer Cell Motility via HGF-MET Pathway Activation in Melanoma  Soonyean Hwang, Hye-Eun Kim, Michelle Min, Rekha.
MiR-223 Regulates Cell Growth and Targets Proto-Oncogenes in Mycosis Fungoides/Cutaneous T-Cell Lymphoma  Laura Y. McGirt, Clare M. Adams, Devin A. Baerenwald,
CD63 Tetraspanin Is a Negative Driver of Epithelial-to-Mesenchymal Transition in Human Melanoma Cells  Antonella Lupia, Silvia Peppicelli, Ewa Witort,
The IL-6 Trans-Signaling-STAT3 Pathway Mediates ECM and Cellular Proliferation in Fibroblasts from Hypertrophic Scar  Sutapa Ray, Xiaoxi Ju, Hong Sun,
Bim Expression Is Reduced in Human Cutaneous Melanomas
Erythroid Differentiation Regulator 1, an Interleukin 18-Regulated Gene, Acts as a Metastasis Suppressor in Melanoma  Min Kyung Jung, Yoorim Park, Seok.
Mark A. Rovedo, Nancy L. Krett, Steven T. Rosen 
Volume 17, Issue 2, Pages (February 2009)
Increased Expression of Wnt2 and SFRP4 in Tsk Mouse Skin: Role of Wnt Signaling in Altered Dermal Fibrillin Deposition and Systemic Sclerosis  Julie Bayle,
MicroRNA-203 Regulates Melanosome Transport and Tyrosinase Expression in Melanoma Cells by Targeting Kinesin Superfamily Protein 5b  Shunsuke Noguchi,
Wynn H. Kao, Adam I. Riker, Deepa S. Kushwaha, Kimberly Ng, Steven A
Jasmine George, Minakshi Nihal, Chandra K
MiRNA Expression Profiling in Melanocytes and Melanoma Cell Lines Reveals miRNAs Associated with Formation and Progression of Malignant Melanoma  Daniel.
Targeted Depletion of Polo-Like Kinase (Plk) 1 Through Lentiviral shRNA or a Small- Molecule Inhibitor Causes Mitotic Catastrophe and Induction of Apoptosis.
HGF-Promoted Motility in Primary Human Melanocytes Depends on CD44v6 Regulated via NF-kappa B, Egr-1, and C/EBP-beta  Sabine Damm, Petra Koefinger, Martina.
miR-330-5p Targets Tyrosinase and Induces Depigmentation
Macrophage Inhibitory Cytokine-1 Is Overexpressed in Malignant Melanoma and Is Associated with Tumorigenicity  Glen M. Boyle, Julie Pedley, Adam C. Martyn,
Anna Flammiger, Robert Besch, Anthony L. Cook, Tanja Maier, Richard A
IL-17A Upregulates Keratin 17 Expression in Keratinocytes through STAT1- and STAT3- Dependent Mechanisms  Xiaowei Shi, Liang Jin, Erle Dang, Ting Chang,
AIM1 and LINE-1 Epigenetic Aberrations in Tumor and Serum Relate to Melanoma Progression and Disease Outcome  Sojun Hoshimoto, Christine T. Kuo, Kelly.
MiR-409 Inhibits Human Non-Small-Cell Lung Cancer Progression by Directly Targeting SPIN1  Qi Song, Quanbo Ji, Jingbo Xiao, Fang Li, Lingxiong Wang, Yin.
Volume 24, Issue 10, Pages (October 2016)
Role and Regulation of STAT3 Phosphorylation at Ser727 in Melanocytes and Melanoma Cells  Masanobu Sakaguchi, Masahiro Oka, Tetsushi Iwasaki, Yasuo Fukami,
Presentation transcript:

B7-H3 Associated with Tumor Progression and Epigenetic Regulatory Activity in Cutaneous Melanoma  Jinhua Wang, Kelly K. Chong, Yoshitaka Nakamura, Linhda Nguyen, Sharon K. Huang, Christine Kuo, Wang Zhang, Hua Yu, Donald L. Morton, Dave S.B. Hoon  Journal of Investigative Dermatology  Volume 133, Issue 8, Pages 2050-2058 (August 2013) DOI: 10.1038/jid.2013.114 Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 B7-H3 messenger RNA (mRNA) expression in melanomas. (a) B7-H3 mRNA expression in American Joint Committee on Cancer stage I, II, III primary melanomas, stage III, IV metastatic melanomas, and nevi/benign lentigo melanocytes/normal skin tissues. (b) B7-H3 mRNA expression in primary melanomas, metastatic melanomas, and nevi/benign lentigo melanocyte/normal skin tissues. Horizontal bars indicate mean relative B7-H3 copy numbers. Journal of Investigative Dermatology 2013 133, 2050-2058DOI: (10.1038/jid.2013.114) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Immunohistochemistry (IHC) staining of B7-H3 of melanomas. (a–d) Representative IHC staining of B7-H3. (a) Negative control (primary antibody isotype control). (b) Nevus. (c) Primary melanomas. (d) Metastatic melanomas. (e) B7-H3 expression (IHC score) in primary melanoma American Joint Committee on Cancer (AJCC) stage II, III, metastatic melanoma AJCC stage III, IV, and nevus. (f) B7-H3 IHC score in primary melanomas, metastatic melanomas, and nevus. Bar=100μm. Journal of Investigative Dermatology 2013 133, 2050-2058DOI: (10.1038/jid.2013.114) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Melanoma-,specific survival (MSS) in stage III and stage IV melanoma (tissue microarray, TMA) patients. (a) Representative TMA immunohistochemistry (IHC) staining of B7-H3. (a1) Lymph node (LN) metastasis with absent expression of B7-H3. (a2) LN metastasis with weak expression of B7-H3. (a3) LN metastasis with moderate expression of B7-H3. (a4) LN metastasis with strong expression of B7-H3. In figures a1–a4, bars indicate 200μm in length. Original magnification × 100. (b) Stage III TMA (n=139) MSS comparing B7-H3 IHC score of 0–1 (no–weak) versus 2–3 (moderate–strong). (c) Stage IV TMA (n=169) MSS comparing B7-H3 IHC score of 0–2 (no–moderate) versus 3 (strong). (d) Analysis of B7-H3 IHC score between paired matched stage III LN metastases and stage IV distant metastases from the same patient in the stage IV TMA. Horizontal lines represent average B7-H3 score among each stage. Journal of Investigative Dermatology 2013 133, 2050-2058DOI: (10.1038/jid.2013.114) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 B7-H3 expression in melanoma cells. (a) Immunohistochemistry (IHC) staining of a melanoma cell line (M14). Top left: bright field; top right: B7-H3; bottom left: 4′,6-diamidino-2-phenylindole (DAPI); bottom right: merged DAPI+B7-H3. Original magnification × 400. (b) Flow cytometry analysis of B7-H3 expression (open histogram) versus antibody isotype controls (shaded histogram) in melanoma cell lines and normal peripheral blood leukocytes (PBL). (c) Western blotting analysis of melanoma cell lines and tissues (T1–T3). Bar=100μm. Journal of Investigative Dermatology 2013 133, 2050-2058DOI: (10.1038/jid.2013.114) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 MiR-29c regulated B7-H3 expression. (a) Binding site of miR-29c (bottom strand) to B7-H3 messenger RNA (mRNA) 3′ untranslated region (UTR) position from 1,338 to 1,346 (top strand; 9bp in length). (b) B7-H3 immunohistochemistry (IHC) staining score versus miR-29c mRNA expression in melanoma tissue microarray samples (paired t-test analysis). (c) Flow cytometry analysis of B7-H3 expression in a melanoma cell line with transfection of miR-29c mimic and with a negative miRNA control (Cntl). (d) Quantitation of B7-H3 expression change after transfection with miR-29c. (e) Relationship of IHC staining score of phosphorylated signal transducer and activator of transcription-3 (p-STAT3) versus B7-H3 in metastatic melanoma paraffin-embedded archival tissue. (f) Western blot of STAT3 and p-STAT3 in a melanoma cell line transfected with miR-29c mimic and with negative miRNA Cntl. (g) Western blot of STAT3, p-STAT3, and cyclin D1 in a melanoma cell lines transfected with B7-H3 small hairpin RNA (shRNA) or with shRNA Cntl. Journal of Investigative Dermatology 2013 133, 2050-2058DOI: (10.1038/jid.2013.114) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 B7-H3 functional studies. (a) Immunoblots of B7-H3 expression in melanoma lines untreated (left), M14 Cntl (Control) versus M14 B7-H3 small hairpin RNA (shRNA) cells (middle), and M219 vector versus M219 B7-H3 cells (right). (b) Migration assay of M14 Cntl versus M14 B7-H3shRNA cells (top) and M219 vector versus M219 B7-H3 cells (bottom). (c) Invasion assay of M14 Cntl versus M14 B7-H3shRNA cells (top) and M219 vector versus M219 B7-H3 cells (bottom). The data were presented as mean±SD (right). (d) Colony formation of M14 Cntl versus M14 B7-H3 shRNA cells (top) and M219 vector versus M219 B7-H3 cells (bottom) in soft agar after 18 days culture. (e) The growth of M14 Cntl versus M14 B7-H3 shRNA cells (top) and M219 vector versus M219 B7-H3 cells (bottom) in three-dimensional Matrigel (M14 Cntl and M14 B7-H3 shRNA refer to cells transfected with shRNA Cntl and B7-H3 shRNA, respectively; M219 vector and M219 B7-H3 refer to cells transfected with empty vector and B7-H3 expression plasmid, respectively). Student’s t-test was used to assess differences between the groups. The data are presented as mean±SD (right). Bar=100μm. Journal of Investigative Dermatology 2013 133, 2050-2058DOI: (10.1038/jid.2013.114) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions