Dahliae-resistant analysis of GbNAC1-silencing (TRV: GbNAC1) and control (TRV: 00) in Xinhai15 (G. barbadense L.). dahliae-resistant analysis of GbNAC1-silencing.

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dahliae-resistant analysis of GbNAC1-silencing (TRV: GbNAC1) and control (TRV: 00) in Xinhai15 (G. barbadense L.). dahliae-resistant analysis of GbNAC1-silencing (TRV: GbNAC1) and control (TRV: 00) in Xinhai15 (G. barbadense L.). (A) The albino phenotype of true leaves after 10 d with the VIGS method. (B) The disease phenotype of TRV: 00 plants and TRV: GbNAC1 plants by inoculation with V. dahliae after 10 d. (C) Diagonal plane of stem by inoculation with V. dahliae after 10 d. (D) RT-PCR analysis of the expression of GbNAC1 in TRV: GbNAC1 and TRV: 00. Cotton gene UBQ7 was used as an internal control. (E) The fungal renewal cultivation of V. dahliae inoculation stem sections on PDA medium. (F) Disease index after inoculation with V. dahliae. PDA, potato dextrose agar; RT-PCR, reverse transcription-polymerase chain reaction; VIGS, virus-induced gene silencing. Weina Wang et al. G3 2016;6:3951-3961 ©2016 by Genetics Society of America